Nucleic Acid Manipulation & Analysis
Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.
RNA sequencing is used to reveal the presence and quantity of RNA in a given sample. In this feed, RNA sequencing investigates the genetic and molecular mechanisms related to the pathophysiology of Alzheimer's disease (AD). Here are the latest discoveries pertaining to RNA sequencing and this disease.
The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.
Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.
Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.
Several sequencing approaches are employed to understand and examine tumor development and progression. These include whole genome as well as RNA sequencing. Here is the latest research on cancer sequencing.
Circulating free DNA (cfDNA) are degraded DNA fragments released to the blood plasma. cfDNA has been shown to be a useful biomarker for a multitude of ailments other than cancer and fetal medicine. Here is the latest research on Circulating free DNA (cfDNA) sequencing.
Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) is a genome-wide technique used to map histone marks and protein binding (ie transcription factors) on chromatin. This can reveal insight into the regulation and dynamics of gene expression. Discover the latest research on ChIP-seq here.
Cancer cells produce circulating tumor DNA (ctDNA) which can be found in the blood stream using novel high-throughput sequencing technologies. Here is the latest research on ctDNA.
Inter/Intra-Species: Comparative Sequencing describes the sequencing techniques that compare between and within species genomes. Discover the latest research on comparative sequencing of inter/intra-species here.
Development of gene editing techniques, including CRISPR and TALENs, has relied on mechanisms that underlie DNA repair and genome stability pathways. This feed focuses on the role of DNA repair mechanisms in gene editing and genetic engineering.
Genetic circuits are essential for many biological processes including oscillation (e.g. the cell cycle), cell differentiation, patterning of multicellular tissues, and cellular response to environmental signals. Here is the latest research on editing these genetic circuits.
Environmental genomics deals with large-scale sequence-based information obtained from environmental samples to link organismal capabilities, organism–environment interactions, functional diversity, ecosystem processes, evolution and Earth history. Discover the latest research on Environmental Genomics here.
Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.
Genome annotation is the process of identifying genes and assigning function to each gene in a genome sequence. It provides the means to elucidate biological function from sequence. Here is the latest research.
Genome editing technologies enable the editing of genes to create or correct mutations or express genes of interest. Here is the latest research on genome editing in T- cells and their application in human diseases such as adoptive T-cell immunotherapy for cancer.
Genome editing is a powerful tool to study specific mutations or genes involved in the development and progression of cardiovascular disease. Discover the latest research on Genome Editing in Cardiovascular Disease here.
Recent developments in genomics and further development of advanced post-genomic technologies, it will be possible to apply a more holistic analysis to genomic analysis. Discover the latest research in Genomic Techniques.
Recent advances in next-generation sequencing have enabled faster and more accurate haplotyping methods to detect variants across a chromosome. Discover the latest research on Haplotyping here.
Infectious Disease Sequencing is a emerging field due to low-cost massively parallel sequencing and allows researchers to monitor outbreaks of infectious pathogens. Discover the latest research on Infectious Disease Sequencing here.
Long non-coding RNA (lncRNA) are regulatory RNAs exceeding 200 nucleotides in length. LncRNAs are implicated in regulation of gene transcription, post-transcriptional regulation, as well as epigenetic epigenetic regulation. Here is the latest research on lncRNAs and cancer.
MicroRNAs are small non-coding RNAs that modulate gene expression at post-transcriptional level, playing a crucial role in cell differentiation and development. Discover the latest research on MicroRNAs & how they target Open Reading Frames.
Nanopore sequencing is a third generation pproach used in the sequencing of biopolymers- specifically, polynucleotides in the form of DNA or RNA. Discover the latest research on nanopore sequencing.
Neuroscience integrates data from anatomical, biological, developmental, and mathematical models to understand the nervous system. Here is the latest research on genomics in neuroscience.
Mutations, single nucleotide variants and large genomic rearrangements, in non-coding regions of the genome can have severe consequences such as disruption of transcription factor binding sites and functions of non-coding RNAs and can impact tumor development and progression. Here is the latest research.
Perturb-seq has combined CRISPR gene inactivation and single-cell rna-sequencing (scRNA-seq) and is the newest addition to the geneticist's arsenal, providing scientists with study methods for functional genomics. Discover the latest research here.
DNA origami uses a long custom single-stranded DNA scaffold to allow template assembly of numerous short oligonucleotides. Filamentous bacteriophages and related phagemids are excellent sources of scaffolds. Here is the latest research on DNA origami and phagemids.
Pharmacogenetics and pharmacogenomics (pgx) are rapidly growing fields that aim to elucidate the genetic basis for the interindividual differences in drug response. Here is the latest research on pharmacogenetics and pharmacogenomics.
Proteogenomics is a multi-omics research field that has the aim to efficiently integrate genomics, transcriptomics and proteomics. Here is the latest research on proteogenomics.
ADAR enzymes are responsible for a-to-i rna editing, an essential post-transcriptional rna modification contributing to transcriptome and proteome diversification.
Genome-wide and high-throughput DNA sequencing in Schizophrenia and Bipolar Disorder can be used to identify genes and pathways that contribute to disease risk. Here is the latest research.
Single cell sequencing (scs) can be harnessed to acquire the genomes, transcriptomes and epigenomes from individual cells. Here is the latest research on single cell sequencing.
Single-cell RNA sequencing is a technique that is used to study an isolated selection of single-cells and obtaining their transcripts which are then used to generate sequence libraries. These can then be used to assess the diversity in the brain and understand neurological diseases. Here is the latest research on single-cell RNA in the brain.
Transcription activator-like effectors (TALEs) and TALE nucleases (TALENs) enable gene editing by targeting specific DNA sequences in the genome. Here is the latest research.
Targeted gene repair is a technique that can be used to change the DNA sequence at a specific site in the genome to either correct or introduce a genetic mutation. Here is the latest research on targeted gene correction.
Transposons can replicate and spread through the host’s genome and therefore be used as a genetic tool for analysis of gene function. Discover the latest research on the use of transposons for gene editing here.
Base editing systems, including those using a uracil DNA glycosylase inhibitor, expand the scope and efficiency of genome editing technologies. The latest research in that area can be found in this feed.
Zinc-finger nucleases are engineered restriction enzymes that enable gene editing by targeting specific DNA sequences in the genome. Here is the latest research.
Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here is the latest research on de novo genome assembly.