Detecting CRISPR Off-Targets

CRISPR-Cas system enables the editing of genes to create or correct mutations. However, its high frequency of off-target activity is a major concern, especially for therapeutic and clinical applications. This feed focuses on methods that can detect CRISPR off-target activity.

February 25, 2021

CARMN Loss Regulates Smooth Muscle Cells and Accelerates Atherosclerosis in Mice.

Circulation Research
Francesca VacanteAndrew H Baker
February 25, 2021

Identification of gene targets of mutant C/EBPα reveals a critical role for MSI2 in CEBPA-mutated AML.

Leukemia
Elizabeth HeyesFlorian Grebien
February 25, 2021
Open Access

C3G Regulates STAT3, ERK, Adhesion Signaling, and Is Essential for Differentiation of Embryonic Stem Cells.

Stem Cell Reviews and Reports
Vijay V VishnuP Chandra Shekar
February 25, 2021

Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq.

Cell Stem Cell
Giandomenico TurchianoToni Cathomen
February 23, 2021
Preprint
Open Access

Improved transformation efficiency of group A Streptococcus by inactivation of a type I restriction modification system

BioRxiv : the Preprint Server for Biology
M. B. FinnMichael R. Wessels
February 24, 2021

EBF1 and PAX5 control pro-B cell expansion via opposing regulation of the Myc gene.

Blood
Rajesh SomasundaramMikael Sigvardsson
February 24, 2021
Open Access

In vivo HSPC gene therapy with base editors allows for efficient reactivation of fetal γ-globin in β-YAC mice.

Blood Advances
Chang LiAndré Lieber
February 24, 2021

Gene editing technology: Towards precision medicine in inherited retinal diseases.

Seminars in Ophthalmology
Brian G BalliosRachel M Huckfeldt
February 23, 2021
Open Access

Non-viral ex vivo genome-editing in mouse bona fide hematopoietic stem cells with CRISPR/Cas9.

Molecular Therapy. Methods & Clinical Development
Suvd ByambaaYutaka Hanazono
February 23, 2021
Open Access

Therapeutic Assessment of Targeting ASNS Combined with l-Asparaginase Treatment in Solid Tumors and Investigation of Resistance Mechanisms.

ACS Pharmacology & Translational Science
Verena ApfelGiorgio G Galli
February 23, 2021
Open Access

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx.

Journal of Visualized Experiments : JoVE
Ruichen SunOmar S Akbari

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