Detecting CRISPR Off-Targets

CRISPR-Cas system enables the editing of genes to create or correct mutations. However, its high frequency of off-target activity is a major concern, especially for therapeutic and clinical applications. This feed focuses on methods that can detect CRISPR off-target activity.

May 7, 2020
ReviewOpen Access

Pathogenomics and Management of Fusarium Diseases in Plants

Sephra N Rampersad
May 23, 2020

Subfamily-specific quantification of endogenous mouse L1 retrotransposons by droplet digital PCR

Analytical Biochemistry
Simon J NewkirkWenfeng An
May 28, 2020

Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing

Cell Reports
Isabel WeisheitDominik Paquet
May 2, 2020
Open Access

Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein

Nucleic Acids Research
Jiexin LiHongsheng Wang
May 6, 2020
Open Access

Local release of siRNA using polyplex-loaded thermosensitive hydrogels

Lies A L FliervoetTina Vermonden
May 8, 2020

Massively parallel CRISPRi assays reveal concealed thermodynamic determinants of dCas12a binding

Proceedings of the National Academy of Sciences of the United States of America
David A SpechtGuillaume Lambert
May 20, 2020

3D Spheroids Propel Tumor Characterization

Trends in Cancer
Shengli LiLeng Han
May 21, 2020

CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Mature Primary Innate Immune Cells

Cell Reports
Luke RigganTimothy E O'Sullivan
May 14, 2020
Open Access

Endogenous CRISPR/Cas9 arrays for scalable whole-organism lineage tracing

James CotterellJames Sharpe
May 27, 2020

Antibody RING-Mediated Destruction of Endogenous Proteins

Molecular Cell
Adel F M IbrahimRonald T Hay
May 22, 2020

Chemical footprint of pesticides used in citrus orchards based on canopy deposition and off-target losses

The Science of the Total Environment
Farshad SoheilifardRosalie van Zelm
May 9, 2020
PreprintOpen Access

Computational analysis of ligand dose range thermal proteome profiles

N. KurzawaMikhail M Savitski
May 29, 2020

Synergistic CRISPRa-Regulated Chondrogenic ECM Deposition Without Exogenous Growth Factors

Tissue Engineering. Part a
Niloofar FarhangRobert D Bowles
May 7, 2020
ReviewOpen Access

Risk Assessment Considerations for Genetically Modified RNAi Plants: EFSA's Activities and Perspective

Frontiers in Plant Science
Nikoletta PapadopoulouElisabeth Waigmann

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