Detecting CRISPR Off-Targets

CRISPR-Cas system enables the editing of genes to create or correct mutations. However, its high frequency of off-target activity is a major concern, especially for therapeutic and clinical applications. This feed focuses on methods that can detect CRISPR off-target activity.

May 7, 2020
ReviewOpen Access

Pathogenomics and Management of Fusarium Diseases in Plants

Pathogens
Sephra N Rampersad
May 23, 2020

Subfamily-specific quantification of endogenous mouse L1 retrotransposons by droplet digital PCR

Analytical Biochemistry
Simon J NewkirkWenfeng An
May 28, 2020

Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing

Cell Reports
Isabel WeisheitDominik Paquet
May 2, 2020
Open Access

Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein

Nucleic Acids Research
Jiexin LiHongsheng Wang
May 6, 2020
Open Access

Local release of siRNA using polyplex-loaded thermosensitive hydrogels

Nanoscale
Lies A L FliervoetTina Vermonden
May 8, 2020

Massively parallel CRISPRi assays reveal concealed thermodynamic determinants of dCas12a binding

Proceedings of the National Academy of Sciences of the United States of America
David A SpechtGuillaume Lambert
May 20, 2020

3D Spheroids Propel Tumor Characterization

Trends in Cancer
Shengli LiLeng Han
May 21, 2020

CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Mature Primary Innate Immune Cells

Cell Reports
Luke RigganTimothy E O'Sullivan
May 14, 2020
Open Access

Endogenous CRISPR/Cas9 arrays for scalable whole-organism lineage tracing

Development
James CotterellJames Sharpe
May 27, 2020

Antibody RING-Mediated Destruction of Endogenous Proteins

Molecular Cell
Adel F M IbrahimRonald T Hay
May 22, 2020

Chemical footprint of pesticides used in citrus orchards based on canopy deposition and off-target losses

The Science of the Total Environment
Farshad SoheilifardRosalie van Zelm
May 9, 2020
PreprintOpen Access

Computational analysis of ligand dose range thermal proteome profiles

bioRxiv
N. KurzawaMikhail M Savitski
May 29, 2020

Synergistic CRISPRa-Regulated Chondrogenic ECM Deposition Without Exogenous Growth Factors

Tissue Engineering. Part a
Niloofar FarhangRobert D Bowles
May 7, 2020
ReviewOpen Access

Risk Assessment Considerations for Genetically Modified RNAi Plants: EFSA's Activities and Perspective

Frontiers in Plant Science
Nikoletta PapadopoulouElisabeth Waigmann

Sign up to follow this feed and discover related papers.

Related Feeds

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Alzheimer's Disease: RNA Sequencing

RNA sequencing is used to reveal the presence and quantity of RNA in a given sample. In this feed, RNA sequencing investigates the genetic and molecular mechanisms related to the pathophysiology of Alzheimer's disease (AD). Here are the latest discoveries pertaining to RNA sequencing and this disease.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Bioconductor Software Package: Human Cell Atlas

The open source software R / Bioconductor is used for statistical analysis and visualization, with continual generation of new analysis packages and updates. Find the latest research on Programming: Bioconductor Software Packages here.

Bioconductor software package (programming)

The open source software R / Bioconductor is used for statistical analysis and visualization, with continual generation of new analysis packages and updates. Find the latest research on programming and Bioconductor here.

© 2020 Meta ULC. All rights reserved
/feed-previews/detecting-crispr-off-targets/e2413f94-91e4-4434-a4ac-d69ab5c3475b