Detecting CRISPR Off-Targets
CRISPR-Cas system enables the editing of genes to create or correct mutations. However, its high frequency of off-target activity is a major concern, especially for therapeutic and clinical applications. This feed focuses on methods that can detect CRISPR off-target activity.
Efficient targeted integration directed by short homology in zebrafish and mammalian cells
Pathogenomics and Management of Fusarium Diseases in Plants
Subfamily-specific quantification of endogenous mouse L1 retrotransposons by droplet digital PCR
Problem Formulation for Off-Target Effects of Externally Applied Double-Stranded RNA-Based Products for Pest Control
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects
Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein
Local release of siRNA using polyplex-loaded thermosensitive hydrogels
Massively parallel CRISPRi assays reveal concealed thermodynamic determinants of dCas12a binding
CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Mature Primary Innate Immune Cells
Endogenous CRISPR/Cas9 arrays for scalable whole-organism lineage tracing
Spatiotemporal Delivery of CRISPR/Cas9 Genome Editing Machinery Using Stimuli-Responsive Vehicles
Antibody RING-Mediated Destruction of Endogenous Proteins
Exploring the Use of Compound-Induced Transcriptomic Data Generated From Cell Lines to Predict Compound Activity Toward Molecular Targets
Specificities and functional coordination between the two Cas6 maturation endonucleases in Anabaena sp. PCC 7120 assign orphan CRISPR arrays to three groups
Chemical footprint of pesticides used in citrus orchards based on canopy deposition and off-target losses
Computational analysis of ligand dose range thermal proteome profiles
Synergistic CRISPRa-Regulated Chondrogenic ECM Deposition Without Exogenous Growth Factors
Risk Assessment Considerations for Genetically Modified RNAi Plants: EFSA's Activities and Perspective
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