Detecting CRISPR Off-Targets

CRISPR-Cas system enables the editing of genes to create or correct mutations. However, its high frequency of off-target activity is a major concern, especially for therapeutic and clinical applications. This feed focuses on methods that can detect CRISPR off-target activity.

July 31, 2020
Open Access

Ex vivo editing of human hematopoietic stem cells for erythroid expression of therapeutic proteins

Nature Communications
Giulia PavaniMario Amendola
August 2, 2020

Screening siRNAs against host glycosylation pathways to develop novel anti-viral agents against hepatitis B virus

Hepatology Research : the Official Journal of the Japan Society of Hepatology
Kiyoaki ItoHisashi Narimatsu
August 14, 2020
Review
Open Access

Progress in Delivery of siRNA-Based Therapeutics Employing Nano-Vehicles for Treatment of Prostate Cancer

Bioengineering
Milad AshrafizadehPooyan Makvandi
August 9, 2020
Review

Current Status and Challenges of DNA Base Editing Tools

Molecular Therapy : the Journal of the American Society of Gene Therapy
You Kyeong JeongSangsu Bae
July 30, 2020
Preprint
Open Access

Small-hairpin RNAs cause target-independent microRNA dysregulation in neurons and elicit global transcriptomic changes

BioRxiv : the Preprint Server for Biology
R. KohenRoman J. Giger
August 11, 2020
Review
Open Access

Ready for Repair? Gene Editing Enters the Clinic for the Treatment of Human Disease

Molecular Therapy. Methods & Clinical Development
Martijn P T ErnstW W M Pim Pijnappel
July 21, 2020
Open Access

Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach

Biological Procedures Online
Tgst LeviOffer Gerlitz
July 30, 2020

Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity

Molecular Cell
Shuai JinCaixia Gao
July 31, 2020
Open Access

AcrIIA5 Suppresses Base Editors and Reduces Their Off-Target Effects

Cells
Mingming LiangZhanjun Li
July 26, 2020
Preprint
Open Access

Effects of individual base-pairs on in vivo target search and destruction kinetics of small RNA

BioRxiv : the Preprint Server for Biology
A. PoddarTaekjip Ha
August 11, 2020
Open Access

CRISPR-Cas9-Mediated In Vivo Gene Integration at the Albumin Locus Recovers Hemostasis in Neonatal and Adult Hemophilia B Mice

Molecular Therapy. Methods & Clinical Development
Qingnan WangYang Yang
July 28, 2020
Preprint
Open Access

A CRISPR/Cas9 vector system for neutrophil-specific gene disruption in zebrafish

BioRxiv : the Preprint Server for Biology
Y. WangQing Deng
July 23, 2020

Genome editing in plants directed by CRISPR/Cas ribonucleoprotein complexes

Yi chuan = Hereditas
Xia LiJian Ping Xu
August 12, 2020

Direct analysis of brain phenotypes via neural blastocyst complementation

Nature Protocols
Hai-Qiang DaiBjoern Schwer
July 21, 2020
Preprint
Open Access

Severe perinatal hydrops fetalis in genome edited pigs with a biallelic five base pair deletion of the Marfan syndrome gene, FBN1

BioRxiv : the Preprint Server for Biology
H.-G. TsangKim M. Summers

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