HDR in Genome Engineering

Homology-directed repair (HDR) is a double-strand break repair pathway that can be exploited to modify genomes in many organisms. Researchers are now using the CRISPR-Cas system to control the specificity of HDR genome engineering techniques. Here is the latest research.

December 7, 2021

Improving CRISPR tools by elucidating DNA repair.

Nature Biotechnology
Jung Min Lim, Hyongbum Henry Kim
December 7, 2021

Species- and site-specific genome editing in complex bacterial communities.

Nature Microbiology
Benjamin E RubinJennifer A Doudna
December 7, 2021

Division of labor between SOS and PafBC in mycobacterial DNA repair and mutagenesis.

Nucleic Acids Research
Oyindamola O AdefisayoMichael S Glickman
December 7, 2021

Caught in motion: human NTHL1 undergoes interdomain rearrangement necessary for catalysis.

Nucleic Acids Research
Brittany L CarrollSylvie Doublié
December 7, 2021

Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.

Nucleic Acids Research
Lindsey M JacksonGeorge-Lucian Moldovan
December 7, 2021

Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination.

Nucleic Acids Research
Jingfei XuHong-Wei Wang
December 7, 2021

VID22 counteracts G-quadruplex-induced genome instability.

Nucleic Acids Research
Elena GalatiFederico Lazzaro
December 6, 2021
Preprint
Open Access

A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression

BioRxiv : the Preprint Server for Biology
C. J. HuangJennifer A Doudna
December 6, 2021
Open Access

Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach.

ELife
Ali SeleitAlexandre Paix
December 6, 2021

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition.

Frontiers in Genome Editing
Jonas Holst WolffJacob Giehm Mikkelsen
December 4, 2021
Review

CRISPR/Cas Technologies and Their Applications in Escherichia coli.

Frontiers in Bioengineering and Biotechnology
Huina DongDawei Zhang
December 4, 2021
Open Access

Effective Genome Editing in Leishmania (Viannia) braziliensis Stably Expressing Cas9 and T7 RNA Polymerase.

Frontiers in Cellular and Infection Microbiology
Caroline R EspadaSilvia R B Uliana
December 2, 2021
Preprint
Open Access

CRISPR gene-drive systems based on Cas9 nickases promote super-Mendelian inheritance in Drosophila

BioRxiv : the Preprint Server for Biology
V. Lopez del AmoValentino Matteo Gantz
December 2, 2021

Cellular heterogeneity in DNA alkylation repair increases population genetic plasticity.

Nucleic Acids Research
Maxence S Vincent, Stephan Uphoff
December 2, 2021

The nuclear and cytoplasmic activities of RNA polymerase III, and an evolving transcriptome for surveillance.

Nucleic Acids Research
Alan C Kessler, Richard J Maraia
December 2, 2021

CRISPR-Associated Transposase System Can Insert Multiple Copies of Donor DNA into the Same Target Locus.

The CRISPR Journal
Junjie YangSheng Yang
December 2, 2021

A Bioluminescence Resonance Energy Transfer-Based Reporter System: Characterization and Applications.

The CRISPR Journal
Tobias WimmerKnut Stieger

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