(-)-Epiafzelechin Protects against Ovariectomy-induced Bone Loss in Adult Mice and Modulate Osteoblastic and Osteoclastic Functions In Vitro

Nutrients
Ka-Chun WongMan-Sau Wong

Abstract

The present study was designed to characterize the bone protective effects of (-)-epiafzelechin (EAF), a flavan-3-ol, in mature ovariectomized mice model and its ability to stimulate osteoblastic activity and inhibit osteoclastic activity. Mature C57BL/6 mice (three to four months old) were either ovariectomised (OVX) or sham-operated and subjected to treatment (vehicle, 17β-oestradiol (E2, 200 μg/kg/day) or EAF (500 μg/kg/day) orally for six weeks. EAF and E2 significantly reduced urinary calcium (Ca) excretion, serum osteocalcin (OCN), and urinary deoxy-pyridinoline (DPD); increased bone mineral density (BMD); and improved micro-architectural properties in OVX mice. EAF significantly increased cell viability, alkaline phosphatise (ALP) activity, and collagen content, as well as runt-related transcriptional factor 2 (Runx2) mRNA expression in murine osteoblastic MC3T3-E1 cells. In addition, EAF significantly reduced the viability of osteoclast precursor murine leukemia monocyte RAW 264.7 cells and tartrate-resistant acid phosphatase (TRAP) activities in mature osteoclastic RAW 264.7 cells. EAF is a bioactive flavan-3-ol that protects estrogen deficiency-induced bone loss in OVX mice and exerts direct modulating effects in bone...Continue Reading

References

Jun 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L D QuarlesR J Wenstrup
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D WangR T Franceschi
Dec 6, 2000·Seminars in Cell & Developmental Biology·G Karsenty
Nov 20, 2001·Endocrinology·S Khosla
Apr 4, 2003·Biological & Pharmaceutical Bulletin·Eun-Mi Choi, Jae-Kwan Hwang
Sep 20, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Chung-Hwan ChenGwo-Jaw Wang
Sep 5, 2006·In Vitro Cellular & Developmental Biology. Animal·Bethany L V CuetaraKevin P McHugh
Sep 12, 2006·The Journal of Nutritional Biochemistry·Bahareh ValiAhmed El-Sohemy
Oct 6, 2007·Endocrine Reviews·Stephen R Hammes, Ellis R Levin
Aug 25, 2009·Nutrition Research·Chwan-Li ShenJia-Sheng Wang
Apr 24, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Y ZhangM-S Wong
May 5, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Antonia C HardcastleHelen M Macdonald
Jun 21, 2011·Menopause : the Journal of the North American Menopause Society·UNKNOWN North American Menopause Society
Dec 22, 2011·Journal of Pharmacological Sciences·Yoshiomi OkaKatsuji Oguchi
Feb 1, 2012·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·C ZuoX Zhang
May 3, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ailsa WelchAedín Cassidy
Mar 21, 2013·Menopause : the Journal of the North American Menopause Society·Chung-Hwan ChenMei-Ling Ho
Oct 1, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xiaobin ZengLiao Cui
Jul 27, 2014·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Z-Q ZhangY-M Chen
Aug 12, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ka Chun WongTak Hang Chan
Aug 14, 2015·The American Journal of Clinical Nutrition·Gael MyersJonathan M Hodgson
Jan 1, 2012·Journal of Pharmacological Sciences·Yoshiomi OkaKatsuji Oguchi

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Citations

Dec 9, 2020·Trends in Endocrinology and Metabolism : TEM·Daniele BellaviaGianluca Giavaresi
Nov 16, 2018·International Journal of Biological Macromolecules·Youn-Hwan HwangTaesoo Kim

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Methods Mentioned

BETA
nuclear magnetic resonance
urine collection
ELISA
protein assay

Software Mentioned

Prism
SDS

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