(-)-Epicatechin in the prevention of tumor necrosis alpha-induced loss of Caco-2 cell barrier integrity

Archives of Biochemistry and Biophysics
Telma C ContrerasPatricia I Oteiza

Abstract

An increased permeability of the intestinal barrier is proposed as a major event in the pathophysiology of inflammatory bowel diseases (IBD). Tumor necrosis alpha (TNFα) plays a central role in IBD pathogenesis, in part promoting tight function (TJ) barrier dysfunction. Food extracts enriched in (-)-epicatechin (EC) prevent the development or improve the progression of IBD in animal models. This study investigated the capacity of EC to inhibit TNFα-induced permeabilization of Caco-2 cell monolayers, characterizing the underlying mechanisms. Caco-2 cells differentiated into intestinal epithelial cells were incubated in the absence/presence of TNFα, with or without the addition of 0.5-5 μM EC. TNFα triggered cell monolayer permeabilization, decreasing transepithelial electrical resistance (TEER) and increasing the paracellular transport of fluorescein sulfonic acid. The permeabilizing effects of TNFα were not due to Caco-2 cell apoptosis as evaluated by DNA fragmentation, caspase 3 and 9 activation, and cell morphology. EC prevented TNFα-triggered Caco-2 monolayer permeabilization and acted inhibiting the associated: (i) NADPH oxidase (NOX)-mediated increased oxidant production, (ii) NF-κB (IκBα phosphorylation, p50 and RelA nucl...Continue Reading

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Citations

Mar 13, 2016·Archives of Biochemistry and Biophysics·Eleonora CremoniniPatricia I Oteiza
Apr 29, 2016·Clinical Psychopharmacology and Neuroscience : the Official Scientific Journal of the Korean College of Neuropsychopharmacology·Alan C LoganSusan L Prescott
Mar 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fei-Yan FanMin Jiang
Feb 22, 2017·Molecular Nutrition & Food Research·Katherine Gil-CardosoXimena Terra
Jul 26, 2017·Food & Function·Eleonora CremoniniPatricia I Oteiza
Aug 16, 2017·World Journal of Gastroenterology : WJG·Ali SalaritabarAnupam Bishayee
Jan 10, 2018·Journal of the Science of Food and Agriculture·Shaomin ZhaoLiwei Gu
Jan 8, 2020·Nutrients·Carlos González-QuilenXimena Terra
Feb 25, 2020·Critical Reviews in Food Science and Nutrition·Zhihao QuSheng Zhang
Dec 17, 2020·Nutrients·Achille Parfait Atchan NwakibanEnrico Sangiovanni
Nov 14, 2020·Expert Review of Clinical Immunology·Yiran YaoJun Shen
Jul 18, 2020·Archives of Biochemistry and Biophysics·Eleonora CremoniniPatricia I Oteiza
Nov 18, 2018·Free Radical Biology & Medicine·Eleonora CremoniniPatricia I Oteiza
May 14, 2018·Toxicon : Official Journal of the International Society on Toxinology·Nan ZhengYanan Gao
Jan 11, 2018·Molecular Aspects of Medicine·P I OteizaD H Taft
Jan 26, 2020·Free Radical Biology & Medicine·Dario E IglesiasPatricia I Oteiza
Apr 4, 2021·Antioxidants·Katarzyna Dziąbowska-GrabiasKrzysztof Sztanke
Feb 25, 2017·Journal of Natural Products·Sandro LuescherVeronika Butterweck
Jul 3, 2019·Journal of Agricultural and Food Chemistry·Stefano BernardiPatrizia Riso

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