(-)-Epigallocatechin-3-Gallate Enhances Hepatitis C Virus Double-Stranded RNA Intermediates-Triggered Innate Immune Responses in Hepatocytes

Scientific Reports
Yizhong WangWenzhe Ho

Abstract

(-)-Epigallocatechin-3-gallate (EGCG), a major polyphenol component of green tea, has recently been identified as an inhibitor of hepatitis C virus (HCV) entry. Here, we examined whether EGCG can enhance hepatocyte-mediated intracellular innate immunity against HCV. HCV dsRNAs (Core, E1-P7, NS-3'NTR and NS5A) induced interferon-λ1 (IFN-λ1) expression in human hepatocytes. These HCV dsRNAs also induced the expression of Toll-like receptor 3 (TLR3), retinoic acid-inducible gene I (RIG-I) and several antiviral IFN-stimulated genes (ISGs) expression. Although EGCG treatment of hepatocytes alone had little effect on TLR3 and RIG-I signaling pathways, EGCG significantly enhanced HCV dsRNAs-induced the expression of IFN-λ1, TLR3, RIG-I and antiviral ISGs in hepatocytes. Furthermore, treatment of HCV-infected hepatocytes with EGCG and HCV dsRNAs inhibited viral replication. Given that EGCG has the ability to enhance HCV dsRNAs-induced intracellular antiviral innate immunity against HCV, suggesting the potential application of EGCG as a new anti-HCV agent for HCV therapy.

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Citations

Feb 6, 2018·Clinical and Experimental Vaccine Research·Jae-Min Song
Aug 10, 2017·BioMed Research International·Andreia GranjaSalette Reis
Nov 17, 2020·Frontiers in Immunology·Pritom Chowdhury, Anoop Kumar Barooah
Feb 27, 2021·Scientific Reports·Gamal ShihaShaker A Mousa
Mar 7, 2021·International Journal of Molecular Sciences·Ji Young SongYoung Min Park
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Methods Mentioned

BETA
transfection
ELISA
PCR

Software Mentioned

MyiQ

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