(-)-Epigallocatechin gallate reduces transforming growth factor beta-stimulated HSP27 induction through the suppression of stress-activated protein kinase/c-Jun N-terminal kinase in osteoblasts

Life Sciences
Kana HayashiO Kozawa

Abstract

We previously reported that transforming growth factor-beta (TGF-beta) stimulates heat shock protein 27 (HSP27) induction through p38 mitogen-activated protein (MAP) kinase and extracellular signal-regulated kinase 1/2 (ERK1/2) in osteoblast-like MC3T3-E1 cells. In the present study, we investigated whether (-)-epigallocatechin gallate (EGCG), the major polyphenol found in green tea, affects the TGF-beta-stimulated induction of HSP27 in these cells, and its underlying mechanism. EGCG significantly suppressed the HSP27 induction stimulated by TGF-beta in a dose-dependent manner between 10 and 30 microM without affecting the HSP70 levels. TGF-beta with or without EGCG did not affect the advanced oxidation protein products. The TGF-beta-induced phosphorylation of p38 MAP kinase and ERK1/2 was not affected by EGCG. SP600125, a specific inhibitor of stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK), markedly reduced the HSP27 expression induced by TGF-beta. EGCG significantly suppressed the TGF-beta-induced phosphorylation of SAPK/JNK without affecting the phosphorylation of Smad2. EGCG attenuated the phosphorylation of both MKK4 and TAK1 induced by TGF-beta. These results strongly suggest that EGCG suppresses the...Continue Reading

References

Sep 15, 1986·Biochemical Pharmacology·J M DelaisséG Vaes
Oct 1, 1986·Physiological Reviews·P J NijweideJ H Feyen
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Jun 5, 1997·Nature·J JankunE Skrzypczak-Jankun
Aug 1, 1998·Circulation Research·I J Benjamin, D R McMillan
Feb 24, 2000·Biochimica Et Biophysica Acta·L F CooperZ Guo
Mar 10, 2000·Cytokine & Growth Factor Reviews·K Miyazono
Jul 6, 2000·Advances in Immunology·K MiyazonoC H Heldin
Dec 29, 2000·Phytochemistry·J B Harborne, C A Williams
Apr 25, 2001·Journal of Cellular Physiology·K MiyazonoH Inoue
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·B L BennettD W Anderson
Apr 4, 2003·Biological & Pharmaceutical Bulletin·Eun-Mi Choi, Jae-Kwan Hwang
May 23, 2003·The Journal of International Medical Research·U UllmannP Weber
Oct 2, 2003·The Journal of Nutrition·Joshua D Lambert, Chung S Yang
May 8, 2004·Antioxidants & Redox Signaling·Imtiaz A SiddiquiHasan Mukhtar
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Orhan AktasFrauke Zipp
Dec 8, 2004·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·U UllmannP Weber
Apr 13, 2005·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Y KannoO Kozawa
Aug 10, 2007·Seminars in Cancer Biology·Jihyeung JuChung S Yang
May 27, 2008·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H TokudaO Kozawa
Apr 27, 2012·Physiological Reviews·John M Kyriakis, Joseph Avruch

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Citations

Nov 26, 2011·Cell Stress & Chaperones·Eunna Chung, Marissa Nichole Rylander
Jun 7, 2014·Journal of Inflammation·Helen VesterClaudine Seeliger
Jan 10, 2012·Nutrition Reviews·Jameela BanuGabriel Fernandes
Aug 1, 2009·Behavioural Brain Research·Anand Kamal SachdevaKanwaljit Chopra
Jan 12, 2019·Critical Reviews in Food Science and Nutrition·Penghui LiZhonghua Liu
Nov 1, 2013·The American Journal of Clinical Nutrition·Chwan-Li ShenJia-Sheng Wang
Mar 24, 2011·Endocrinology·Kenji KatoTakanobu Otsuka
Sep 14, 2017·International Journal of Molecular Medicine·Shingo KainumaTakanobu Otsuka
Nov 20, 2020·Antioxidants·Hsuan-Ti HuangChwan-Li Shen
Nov 20, 2009·Journal of Agricultural and Food Chemistry·Anna TrzeciakiewiczMarie-Noelle Horcajada
Aug 25, 2009·Nutrition Research·Chwan-Li ShenJia-Sheng Wang
Apr 9, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Chwan-Li ShenJia-Sheng Wang

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