β-1,3/1,4-Glucan Lichenan from Cetraria islandica (L.) ACH. induces cellular differentiation of human keratinocytes

Fitoterapia
Dominika M ZacharskiAndreas Hensel

Abstract

Lichenan (molecular weight 275 kDa, β-D-1,3/1,4-glucopyranose ratio 1:3) from Cetraria islandica at a concentration of 100 μg/mL induced terminal cellular differentiation of primary human keratinocytes as demonstrated by immunofluorescence staining using cytokeratin 10 and involucrin as marker proteins. Lichenan-derived oligosaccharides (DP3 to 8), obtained by acid-catalyzed partial hydrolysis of the polymer, did not influence cellular differentiation. Cytokeratin, filaggrin, involucrin, loricrin and transglutaminase gene expression as typical differentiation markers was increased by lichenan in a time-dependent manner. Lichenan upregulated gene cluster which were mostly related to cellular differentiation with focus on MAPK signaling as was shown by Whole Human Genome Microarray. These gene expression data from the array experiments were subsequently confirmed by qPCR for selected genes. For identification of the molecular binding structures of lichenan 1- and 2-D PAGE of keratinocyte protein membrane preparations was performed, followed by blotting with FITC-labeled lichenan and subsequent mass spectrometric identification of the pinpointed proteins. Epidermal growth factor receptor (EGFR) and integrin β4, both proteins being...Continue Reading

Citations

Feb 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stefan EschAndreas Hensel
Mar 6, 2021·International Journal of Biological Macromolecules·Lijuan WuChenyang Zhao
Apr 6, 2021·Critical Reviews in Food Science and Nutrition·Karthika MuthuramalingamMoonjae Cho
Dec 31, 2021·Journal of the American Society for Mass Spectrometry·Hou-Yu Lin, Chi-Kung Ni

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