β-1,6-linked Galactofuranose- rich peptidogalactomannan of Fusarium oxysporum is important in the activation of macrophage mechanisms and as a potential diagnostic antigen

Medical Mycology
Nathalia Ferreira de OliveiraEliana Barreto-Bergter

Abstract

A peptidogalactomannan (PGM) from Fusarium oxysporum was structurally characterized by a combination of chemical and spectroscopic methods, including one and two-dimensional nuclear magnetic resonance (1D and 2D NMR). The galactomannan component consists of a main chain containing (1→6)-linked β-D-galactofuranose residues with side chains containing (1→2)-linked α-D-Glcp, (1→2)-linked -β-D-Manp (1→2) and β-D-Manp terminal nonreducing end units and differs from that of Aspergillus fumigatus and Cladosporium resinae that present a main chain containing (1→6)-linked α-D-Manp residues presenting β-D-Galf as side chains of 3-4 units that are (1→5)-interlinked. The importance of the carbohydrate moiety of the F. oxysporum PGM was demonstrated. Periodate oxidation abolished much of the PGM antigenic activity. A strong decrease in reactivity was also observed with de-O-glycosylated PGM. In addition, de-O-glycosylated PGM was not able to inhibit F. oxysporum phagocytosis, suggesting that macrophages recognize and internalize F. oxysporum via PGM. F. oxysporum PGM triggered TNF-α release by macrophages. Chemical removal of O-linked oligosaccharides from PGM led to a significant increase of TNF-α cytokine levels, suggesting that their rem...Continue Reading

References

Jan 1, 1992·Current Topics in Medical Mycology·E AnaissieM Rinaldi
Sep 1, 1993·Journal of Clinical Microbiology·R Kappe, A Schulze-Berge
Sep 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J Guarro, J Gené
Jan 1, 1997·Journal of Clinical Microbiology·C M SwaninkP E Verweij
Jan 8, 1999·Biochimica Et Biophysica Acta·M LussierH Bussey
Mar 13, 1999·Medical Mycology·R M HaidoE Barreto Bergter
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Sep 26, 2003·Current Microbiology·Maria Cleide Florentino da Silva BahiaEliana Barreto-Bergter
Jun 13, 2006·The Journal of Biological Chemistry·Vera Carolina B BittencourtEliana Barreto-Bergter
Jul 31, 2007·Diagnostic Microbiology and Infectious Disease·Jessica R CummingsRandall T Hayden
Oct 16, 2007·Clinical Microbiology Reviews·Marcio Nucci, Elias Anaissie
Feb 6, 2009·Medical Mycology·Andréa R Bernardes-EngemannLeila M Lopes-Bezerra
Oct 21, 2010·The Journal of Biological Chemistry·Rodrigo T FigueiredoMarcelo T Bozza
Feb 10, 2011·Virulence·Maged MuhammedEleftherios Mylonakis
Apr 16, 2011·The Journal of Antimicrobial Chemotherapy·Josep Guarro
Dec 30, 2011·Journal of Clinical Microbiology·Anna Maria TortoranoGiuliana Lo Cascio
Jul 28, 2012·Journal of Proteome Research·ZhenYu ZhuYiFeng Chai
Aug 13, 2013·Clinics in Laboratory Medicine·Audrey N Schuetz
Aug 24, 2013·Journal of Clinical Microbiology·Elitza S TheelMatthew J Binnicker
Oct 15, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M NucciE Anaissie
May 31, 2016·International Journal of Medical Microbiology : IJMM·Annegret WiedemannFrank Ebel
Sep 19, 2017·Nature Reviews. Microbiology·Frank L van de VeerdonkJean-Paul Latgé

❮ Previous
Next ❯

Citations

Jun 25, 2019·Medical Mycology·Ana Fernández-CruzPatricia Muñoz
Feb 22, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Giulia Maria Pires Dos SantosMarcia Ribeiro Pinto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.