β-Actin-dependent global chromatin organization and gene expression programs control cellular identity

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Xin XiePiergiorgio Percipalle

Abstract

During differentiation and development, cell fate and identity are established by waves of genetic reprogramming. Although the mechanisms are largely unknown, during these events, dynamic chromatin reorganization is likely to ensure that multiple genes involved in the same cellular functions are coregulated, depending on the nuclear environment. In this study, using high-content screening of embryonic fibroblasts from a β-actin knockout (KO) mouse, we found major chromatin rearrangements and changes in histone modifications, such as methylated histone (H)3-lysine-(K)9. Genome-wide H3K9 trimethylation-(Me)3 landscape changes correlate with gene up- and down-regulation in β-actin KO cells. Mechanistically, we found loss of chromatin association by the Brahma-related gene ( Brg)/Brahma-associated factor (BAF) chromatin remodeling complex subunit Brg1 in the absence of β-actin. This actin-dependent chromatin reorganization was concomitant with the up-regulation of sets of genes involved in angiogenesis, cytoskeletal organization, and myofibroblast features in β-actin KO cells. Some of these genes and phenotypes were gained in a β-actin dose-dependent manner. Moreover, reintroducing a nuclear localization signal-containing β-actin i...Continue Reading

References

Jun 5, 2002·Annual Review of Biochemistry·Ivan A OlaveGerald R Crabtree
Apr 28, 2004·Journal of Structural Biology·Takeshi ShimiYasushi Hiraoka
Jul 9, 2004·Molecular Biology of the Cell·B HinzJ-J Meister
Apr 1, 2005·Neoplasia : an International Journal for Oncology Research·Madhuchhanda RoyDario Marchetti
Apr 6, 2005·Human Molecular Genetics·Hugh D MorganWolf Reik
May 19, 2006·Nature Reviews. Genetics·Ivana L de la SernaAnthony N Imbalzano
Jun 6, 2007·Nature Reviews. Genetics·Asifa Akhtar, Susan M Gasser
Dec 7, 2007·Genes & Development·Robert Schneider, Rudolf Grosschedl
Dec 12, 2007·The Journal of Cell Biology·Miroslav DundrA Gregory Matera
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jan 27, 2009·Biochimica Et Biophysica Acta·Andreas Lennartsson, Karl Ekwall
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Randall S Gieni, Michael J Hendzel
May 7, 2010·Current Opinion in Cell Biology·Jop Kind, Bas van Steensel
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Neus Visa, Piergiorgio Percipalle
Aug 7, 2010·Advances in Experimental Medicine and Biology·Zbigniew DarzynkiewiczHong Zhao
Nov 19, 2010·Cold Spring Harbor Perspectives in Biology·Martin Alexander Schwartz
Feb 8, 2011·Journal of Cardiovascular Pharmacology·Jennifer Baum, Heather S Duffy
Sep 9, 2011·Molecular Biology of the Cell·Tina M BunnellJames M Ervasti
Mar 27, 2012·Molecular & Cellular Proteomics : MCP·Davina TondeleirChristophe Ampe
Jan 1, 2013·Cellular and Molecular Life Sciences : CMLS·Kei Miyamoto, J B Gurdon
Nov 20, 2013·Trends in Cell Biology·Prabodh Kapoor, Xuetong Shen
Dec 10, 2013·Nature Structural & Molecular Biology·Stephanie A MorrisGordon L Hager
Dec 25, 2013·Nature Reviews. Genetics·Taiping Chen, Sharon Y R Dent
Mar 12, 2015·Nature Reviews. Molecular Cell Biology·Ana Pombo, Niall Dillon
Mar 20, 2015·Epigenetics & Chromatin·Nehmé SaksoukJérôme Déjardin
Mar 31, 2015·Development·Jeffrey M AlexanderBenoit G Bruneau
May 17, 2015·The Journal of Biological Chemistry·Andreas HerchenhanMichael Kjaer
Dec 15, 2015·Cell Host & Microbe·Shashank TripathiSumit K Chanda
Mar 5, 2016·International Review of Cell and Molecular Biology·Lidija D VukovićDaniel L Levy
Apr 30, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bader AlmuzzainiPiergiorgio Percipalle
Jun 28, 2016·Scientific Reports·Leonid A SerebryannyyPrimal de Lanerolle

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Citations

Oct 13, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Dylane M WinelandTina L Tootle
Oct 13, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Daniel J Kelpsch, Tina L Tootle
Apr 12, 2019·Genesis : the Journal of Genetics and Development·Tammy Y CarterJeffrey H Thomas
Sep 5, 2019·Nature Cell Biology·Christopher Patrick CaridiIrene Chiolo
Jul 19, 2018·Histochemistry and Cell Biology·Csaba BajuszPéter Vilmos
Jul 16, 2019·Molecular Biology of the Cell·Piergiorgio Percipalle, Maria Vartiainen
Nov 11, 2020·Journal of Biochemistry·Nanako MachidaMasahiko Harata
Dec 23, 2020·Journal of Biochemistry·Tomas VenitPiergiorgio Percipalle
Jan 23, 2021·Journal of Biochemistry·Kei Miyamoto, Masahiko Harata
Dec 9, 2020·Cold Spring Harbor Perspectives in Biology·Svenja UlfertsMaria K Vartiainen
Jan 20, 2020·Analytical Biochemistry·M A El-SamahyFeng Wang
Apr 24, 2021·The FEBS Journal·Junko Tomikawa, Kei Miyamoto
Aug 7, 2021·Brain Sciences·Tamara Lapeña-LuzónPilar Gonzalez-Cabo
Sep 4, 2021·Nature Communications·Syed Raza MahmoodPiergiorgio Percipalle
Jul 2, 2020·Cell Reports·Tomomi OkunoKei Miyamoto

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