β-Adrenergic receptor agonist, compound 49b, inhibits TLR4 signaling pathway in diabetic retina

Immunology and Cell Biology
Elizabeth A BergerJena J Steinle

Abstract

Diabetic retinopathy has recently become associated with complications similar to chronic inflammatory diseases. Although it is clear that tumor necrosis factor-α is increased in diabetes, the role of innate immunity is only recently being investigated. As such, we hypothesized that diabetes would increase Toll-like receptor 4 (TLR4) signaling, which could be inhibited by a β-adrenergic receptor agonist (Compound 49b) previously shown to have anti-inflammatory actions. In order to investigate β-adrenergic receptor signaling and TLR4 in the diabetic retina, streptozotocin-injected diabetic mice, as well as human primary retinal endothelial cells (RECs) and rat retinal Müller cells (rMC-1) exposed to high glucose (25 mM), were treated with a novel β-adrenergic receptor agonist, Compound 49b (50 nM), or phosphate-buffered saline (control). TLR4 and its downstream signaling partners (MyD88, IL-1 receptor-associated kinase 1, TNF receptor-associated factor 6 and total and phosphorylated nuclear factor-κB) were examined. In addition, we assessed high-mobility group box 1 (HMGB1) protein levels. Our data showed that diabetes or high-glucose culture conditions significantly increased TLR4 and downstream signaling partners. Compound 49b...Continue Reading

References

Jan 1, 1990·Journal of Ocular Pharmacology·P P ElenaP Lapalus
Jan 6, 2001·The American Journal of Pathology·A M JoussenA P Adamis
Jan 31, 2004·Seminars in Immunology·Kiyoshi Takeda, Shizuo Akira
Jul 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonia M JoussenAnthony P Adamis
Sep 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yunpeng DuT S Kern
Jul 1, 2011·Investigative Ophthalmology & Visual Science·Jia YinFu-Shin X Yu
Nov 24, 2011·Investigative Ophthalmology & Visual Science·Robert J WalkerJena J Steinle
Jan 24, 2012·Cellular Signalling·Youde JiangJena J Steinle
Apr 3, 2012·Investigative Ophthalmology & Visual Science·Qiuhua ZhangJena J Steinle
Dec 5, 2012·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Xiaomin LinShao Bo Su
Jul 23, 2013·Investigative Ophthalmology & Visual Science·Qiuhua ZhangJena J Steinle
Jul 23, 2013·PloS One·Jie TangTimothy S Kern
Dec 12, 2013·Journal of Neuroinflammation·Steven F AbcouwerDavid A Antonetti
Jun 18, 2014·Journal of Internal Medicine·A TsungT R Billiar
Nov 2, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yan-Ling WangJian-You Guo
Jan 31, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jichun WangHong Jiang

❮ Previous
Next ❯

Citations

Nov 28, 2018·Journal of Cellular Physiology·Mahbobeh SatariZatollah Asemi
Jun 14, 2018·Experimental and Therapeutic Medicine·Xiaolei TangGuirong Li
Aug 16, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Lili HuShuanghong Jiang
Jul 7, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Li LiuJena J Steinle
Oct 8, 2020·Ocular Immunology and Inflammation·Karanvir S Raman, Joanne A Matsubara
Apr 1, 2021·Experimental Eye Research·Adam SeidelJena J Steinle
Sep 10, 2021·Molecular and Cellular Biochemistry·Li LiuJena J Steinle

❮ Previous
Next ❯

Software Mentioned

Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.