β-Amyloid induces nuclear protease-mediated lamin fragmentation independent of caspase activation

Biochimica Et Biophysica Acta
Vijay Sankar RamasamyIl-Seon Park

Abstract

β-Amyloid (Aβ), a hallmark peptide of Alzheimer's disease, induces both caspase-dependent apoptosis and non-apoptotic cell death. In this study, we examined caspase-independent non-apoptotic cell death preceding caspase activation in Aβ42-treated cells. We first determined the optimal treatment conditions for inducing cell death without caspase activation and selected a double-treatment method involving the incubation of cells with Aβ42 for 4 and 6 h (4+6 h sample). We observed that levels of lamin A (LA) and lamin B (LB) were reduced in the 4+6 h samples. This reduction was decreased by treatment with suc-AAPF-CMK, an inhibitor of nuclear scaffold (NS) protease, but not by treatment with z-VAD-FMK, a pan-caspase inhibitor. In addition, suc-AAPF-CMK decreased the changes in nuclear morphology observed in cells in the 4+6 h samples, which were different from nuclear fragmentation observed in STS-treated cells. Furthermore, suc-AAPF-CMK inhibited cell death in the 4+6 h samples. LA and LB fragmentation occurred in the isolated nuclei and was also inhibited by suc-AAPF-CMK. Together, these data indicated that the fragmentation of LA and LB in the Aβ42-treated cells was induced by an NS protease, whose identity is not clearly deter...Continue Reading

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y A LazebnikW C Earnshaw
Apr 11, 1997·The Journal of Biological Chemistry·R V TalanianW W Wong
Apr 7, 1997·Biochemical and Biophysical Research Communications·B ZhivotovskyS Orrenius
Jul 31, 1999·The Journal of Biological Chemistry·C ScaffidiM E Peter
Oct 21, 1999·Neurobiology of Disease·K J IvinsC W Cotman
May 5, 2004·The Journal of Cell Biology·Junichi HitomiMasaya Tohyama
Jul 24, 2004·Nature Medicine·Ella Bossy-WetzelStuart A Lipton
Dec 21, 2004·Nature Neuroscience·James P ClearyKaren H Ashe
Feb 4, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mark WogulisRussell E Rydel
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Il-Seon Park, Ja-Eun Kim
Sep 29, 2006·Journal of Cellular Biochemistry·Chetan DhamneGary A Clawson
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jan 5, 2008·Cell Death and Differentiation·L Vande WalleP Vandenabeele
Oct 29, 2008·Oncogene·P Boya, G Kroemer
Mar 28, 2009·Cell Death and Differentiation·S InoueG M Cohen
Oct 27, 2009·Biochimica Et Biophysica Acta·Paula I MoreiraGeorge Perry
Apr 24, 2010·Human Molecular Genetics·Leslie Crews, Eliezer Masliah
Mar 11, 2011·Molecular Biology of the Cell·Kuei-Hua ChangKavita Shah
Aug 20, 2011·Nature Reviews. Drug Discovery·Eric KarranBart De Strooper
Dec 6, 2011·PloS One·Dagmar E EhrnhoeferMichael R Hayden
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Christian HaassSangram Sisodia
May 15, 2012·Cardiovascular Research·Matthew A Smith, Rick G Schnellmann
May 19, 2012·Nature Reviews. Neuroscience·Bradley T Hyman, Junying Yuan
Sep 27, 2012·BMB Reports·Eun-Woo LeeJaewhan Song
Apr 3, 2013·Cold Spring Harbor Perspectives in Biology·David R McIlwainTak W Mak
Jun 5, 2013·Cold Spring Harbor Perspectives in Biology·Amanda B ParrishSally Kornbluth
Jan 16, 2014·Biochimica Et Biophysica Acta·Md Golam SharoarIl-Seon Park
Jul 22, 2014·Alzheimer's Research & Therapy·William F GoureFranz Hefti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.