α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart

Development
Alexia ViteGlenn L Radice

Abstract

Shortly after birth, muscle cells of the mammalian heart lose their ability to divide. At the same time, the N-cadherin/catenin cell adhesion complex accumulates at the cell termini, creating a specialized type of cell-cell contact called the intercalated disc (ICD). To investigate the relationship between ICD maturation and proliferation, αE-catenin (Ctnna1) and αT-catenin (Ctnna3) genes were deleted to generate cardiac-specific α-catenin double knockout (DKO) mice. DKO mice exhibited aberrant N-cadherin expression, mislocalized actomyosin activity and increased cardiomyocyte proliferation that was dependent on Yap activity. To assess effects on tension, cardiomyocytes were cultured on deformable polyacrylamide hydrogels of varying stiffness. When grown on a stiff substrate, DKO cardiomyocytes exhibited increased cell spreading, nuclear Yap and proliferation. A low dose of either a myosin or RhoA inhibitor was sufficient to block Yap accumulation in the nucleus. Finally, activation of RhoA was sufficient to increase nuclear Yap in wild-type cardiomyocytes. These data demonstrate that α-catenins regulate ICD maturation and actomyosin contractility, which, in turn, control Yap subcellular localization, thus providing an explanat...Continue Reading

References

Aug 1, 1996·Journal of Molecular and Cellular Cardiology·F LiA M Gerdes
Nov 1, 1996·The American Journal of Physiology·M H SoonpaaL J Field
Oct 13, 2000·American Journal of Physiology. Cell Physiology·H B WangY L Wang
Dec 14, 2002·Science·Kenneth D PossMark T Keating
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Dec 13, 2005·Developmental Biology·Alain HirschyJean-Claude Perriard
Feb 14, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Mark F BerryH Lee Sweeney
Jul 1, 2008·Biophysical Journal·Jeffrey G JacotJeffrey H Omens
Nov 26, 2008·Nature·Adam C MartinEric F Wieschaus
Sep 22, 2009·Current Biology : CB·Eric A KleinRichard K Assoian
Mar 26, 2010·Nature·Chris JoplingJuan Carlos Izpisúa Belmonte
May 11, 2010·Nature Cell Biology·Thomas Lecuit
May 11, 2010·Nature Cell Biology·Shigenobu YonemuraMai Shibata
Dec 22, 2010·Journal of Cell Science·Paul A Janmey, R Tyler Miller
Jan 25, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Anant ChopraJ Yasha Kresh
Feb 26, 2011·Science·Enzo R PorrelloHesham A Sadek
Mar 8, 2011·Cell·Karin SchlegelmilchFernando D Camargo
Jun 4, 2011·PloS One·Justin D MihDaniel J Tschumperlin
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
Aug 13, 2011·Development·Ken-Ichi WadaHiroshi Sasaki
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alexander von GiseWilliam T Pu
Oct 26, 2012·Journal of Cell Science·Justin D MihDaniel J Tschumperlin
Dec 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Enzo R PorrelloHesham A Sadek
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mei XinEric N Olson
Nov 21, 2013·Development·Todd HeallenJames F Martin
May 23, 2014·Current Biology : CB·Stephanie MajkutDennis E Discher
Jul 26, 2014·Annual Review of Cell and Developmental Biology·D E Leckband, J de Rooij
Aug 1, 2014·Nature Communications·Mingxi YaoJie Yan
Aug 8, 2014·Bioarchitecture·Xuefei Ma, Robert S Adelstein
Oct 12, 2014·Circulation Research·Jifen LiGlenn L Radice
Jun 26, 2015·Genes & Development·Toshiro MoroishiKun-Liang Guan

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Citations

Aug 17, 2018·Nature Reviews. Cardiology·Jun WangJames F Martin
Sep 1, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Shijie Liu, James F Martin
Jul 7, 2018·Circulation Research·Ronald J VagnozziSteven R Houser
Apr 28, 2019·Nature Reviews. Cardiology·Karyn M AustinWilliam T Pu
Jun 20, 2020·Nature Reviews. Drug Discovery·Anwesha DeyKun-Liang Guan
Jan 27, 2019·Nature Reviews. Cardiology·Jeffrey J SaucermanJeffrey H Omens
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Yufan LiuXiaobing Fu
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Hamid EsmaeiliJangwook P Jung
Dec 16, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Akane SakaguchiWataru Kimura
May 1, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mingyao SunYuan Luo
Jun 24, 2021·BioMed Research International·Ryan A Behmer HansenSamuel E Senyo
May 11, 2019·ACS Applied Materials & Interfaces·Elise A CorbinKenneth B Margulies
Sep 17, 2021·Cardiovascular Research·Fansen MengJames F Martin

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