α-chymotrypsin activated and stabilized by self-assembled polypseudorotaxane fabricated with bis-thiolated poly(ethylene glycol) and α-cyclodextrin: Spectroscopic and mechanistic analysis

International Journal of Biological Macromolecules
Jun ZhaoMing-Xia Chen

Abstract

The self-assembled polypseudorotaxane (PPRX) fabricated with bis-thiolated poly(ethylene glycol) (PEG) and α-cyclodextrin (α-CyD) acted as an activator for α-chymotrypsin (CT) and retained the activity of CT for a long time up to 7days. The stabilization mechanism was studied, and the interaction between CT and PPRX was analyzed by using circular dichroism, fluorescence spectra and X-ray powder diffraction (XRD). The bis-thiolated PEG and its assembled PPRX with α-CyD exhibited the interaction with the C-terminal region of the CT's B-chain probably through PEGylation of the surface disulfide bridge of CT. It caused the aromatic chromophores more exposed to the hydrophilic microenvironment, leading to conformational variation of CT that was revealed by spectroscopic analysis. It rendered the peptide chains in a more flexible and active state. As a comparison, the non-thiolated components could not decorate the surface of CT and performed almost no effect on its stability, which demonstrated that the decoration of the surface disulfide bridge was a key factor in retaining the activity of CT. Due to the activation and stabilization effect, bis-thiolated PEG/α-CyD PPRX was an excellent soft-immobilized carrier for CT, and provided ...Continue Reading

References

Mar 3, 1975·Biochemical and Biophysical Research Communications·B M Martin, T Viswanatha
Mar 3, 1981·Biochemistry·J P Hennessey, W C Johnson
Jan 23, 1997·Biochemical and Biophysical Research Communications·E VárallyayL Szilágyi
May 23, 2000·Bioconjugate Chemistry·J M MessmoreR T Raines
Oct 24, 2001·The Journal of Organic Chemistry·Hugo E. GottliebAbraham Nudelman
Jun 11, 2002·Biochemical and Biophysical Research Communications·L M SimonI Laczkó
Dec 12, 2002·Chemical Reviews·Lizbeth Hedstrom
Apr 23, 2003·Biochemical and Biophysical Research Communications·M KotormánL M Simon
Apr 6, 2004·Biochemical and Biophysical Research Communications·L M SimonI Laczkó
Sep 15, 2005·Journal of the American Chemical Society·Chang-Cheng YouVincent M Rotello
Mar 9, 2006·Chemical Reviews·Gerhard WenzAxel Müller
Feb 13, 2007·Bioorganic & Medicinal Chemistry Letters·Taishi HigashiKaneto Uekama
Oct 9, 2007·Advanced Drug Delivery Reviews·Steve BrocchiniSunil Shaunak
Apr 10, 2009·The Journal of Physical Chemistry. B·Pannur VenkatesuHo-Mu Lin
May 8, 2009·Chemical Society Reviews·Akira HaradaHiroyasu Yamaguchi
May 19, 2009·International Journal of Pharmaceutics·Taishi HigashiKaneto Uekama
Sep 10, 2009·Chemical Reviews·Akira HaradaYoshinori Takashima
Jan 6, 2010·The Journal of Physical Chemistry. B·Pankaj AttriMing-Jer Lee
Jan 26, 2010·Biochemical and Biophysical Research Communications·Sanjay RawatDebendra K Sahoo
Jan 30, 2010·Chemical Society Reviews·Yong Chen, Yu Liu
Sep 13, 2011·Advanced Drug Delivery Reviews·Tim SernoGerhard Winter
Nov 19, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gianfranco Pasut, Francesco M Veronese
Apr 18, 2012·Chemical Reviews·Awanish Kumar, Pannuru Venkatesu
Sep 22, 2012·Bioconjugate Chemistry·Hanieh KhaliliSteve Brocchini
Jun 4, 2014·Journal of the American Chemical Society·Michael SenskeSimon Ebbinghaus
Dec 17, 2014·Journal of Agricultural and Food Chemistry·Yaping WangLanghong Wang
Dec 24, 2015·Bioconjugate Chemistry·Aleksandra WierzbaDorota Gryko
Jan 1, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Nirnay SamantaMartina Havenith
Feb 6, 2017·The Journal of Physical Chemistry. B·Animesh PatraRajib Kumar Mitra

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Citations

Jan 8, 2020·Materials·Adrian DomińskiPiotr Kurcok
Mar 30, 2018·International Journal of Biological Macromolecules·Sadegh FarhadianAli A Saboury

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