ß-Cyanoalanine Synthase Action in Root Hair Elongation is Exerted at Early Steps of the Root Hair Elongation Pathway and is Independent of Direct Cyanide Inactivation of NADPH Oxidase

Plant & Cell Physiology
Lucía Arenas-AlfonsecaIrene García

Abstract

In Arabidopsis thaliana, cyanide is produced concomitantly with ethylene biosynthesis and is mainly detoxified by the ß-cyanoalanine synthase CAS-C1. In roots, CAS-C1 activity is essential to maintain a low level of cyanide for proper root hair development. Root hair elongation relies on polarized cell expansion at the growing tip, and we have observed that CAS-C1 locates in mitochondria and accumulates in root hair tips during root hair elongation, as shown by observing the fluorescence in plants transformed with the translational construct ProC1:CASC1-GFP, containing the complete CAS-C1 gene fused to green fluorescent protein (GFP). Mutants in the SUPERCENTIPEDE (SCN1) gene, that regulate the NADPH oxidase gene ROOT HAIR DEFECTIVE 2 (RHD2)/AtrbohC, are affected at the very early steps of the development of root hair that do not elongate and do not show a preferential localization of the GFP accumulation in the tips of the root hair primordia. Root hairs of mutants in CAS-C1 or RHD2/AtrbohC, whose protein product catalyzes the generation of ROS and the Ca2+ gradient, start to grow out correctly, but they do not elongate. Genetic crosses between the cas-c1 mutant and scn1 or rhd2 mutants were performed, and the detailed phenoty...Continue Reading

References

Aug 1, 1997·The Plant Journal : for Cell and Molecular Biology·C L WymerS Gilroy
Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·M A TorresJ D Jones
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A B Bleecker, H Kende
Oct 21, 2000·The Plant Cell·J S ParkerC S Grierson
Jun 25, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rachel J Carol, Liam Dolan
Mar 1, 1990·The Plant Cell·J. W. Schiefelbein, C. Somerville
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
May 8, 2004·Trends in Plant Science·Jozef SamajDiedrik Menzel
Jul 15, 2005·Journal of Experimental Botany·Gloria Gutiérrez-AlcaláLuis C Romero
Jul 19, 2005·Journal of Pharmaceutical and Biomedical Analysis·C Charles LinRominder Singh
May 25, 2006·Journal of Experimental Botany·Rachel J Carol, Liam Dolan
Jun 9, 2006·Plant Physiology·Catherine Gapper, Liam Dolan
Nov 14, 2006·Annals of Emergency Medicine·Alan H HallGregory Bogdan
Jan 16, 2007·Phytochemistry·Erich Glawischnig
Feb 16, 2007·Journal of Experimental Botany·Mark A JonesNicholas Smirnoff
May 10, 2007·IUBMB Life·Rita Cipollone, Paolo Visca
Jun 2, 2007·Environment International·D B DonatoB N Noller
Jun 5, 2007·The American Journal of Emergency Medicine·Stephen W BorronChantal Bismuth
Nov 21, 2007·Plant Physiology·Mutsumi WatanabeKazuki Saito
Dec 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·G B MonshausenS Gilroy
Feb 9, 2008·Annual Review of Plant Biology·Tetsuya IshidaTakuji Wada
Oct 10, 2008·Phytochemistry·Markus Piotrowski
Sep 21, 2010·Trends in Plant Science·Marc LibaultGary Stacey
Mar 5, 2013·Plant Signaling & Behavior·Kalaipandian SundaravelpandianWolfgang Schmidt
Nov 29, 2013·Molecular Plant·Luis C RomeroCecilia Gotor
Jan 9, 2014·Plant Signaling & Behavior·Irene GarcíaLuis C Romero
Jan 22, 2014·Current Opinion in Plant Biology·Tijs Ketelaar
Jul 2, 2014·The Arabidopsis Book·Claire GriersonJohn Schiefelbein

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Citations

May 16, 2019·Journal of Experimental Botany·Cecilia GotorLuis C Romero
Nov 17, 2020·Frontiers in Plant Science·Vijay KumarKarl-Josef Dietz
Mar 9, 2021·Frontiers in Plant Science·Dario Di SilvestreIrene Murgia
Mar 27, 2021·Journal of Experimental Botany·Lucía Arenas-AlfonsecaIrene García
Oct 24, 2021·Journal of Experimental Botany·Samuel Gámez-ArcasJavier Pozueta-Romero

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