β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy

Biochimica Et Biophysica Acta. Molecular Cell Research
Xingxing ShiJing Zhao

Abstract

Cardiac stem cells (CSCs) have emerged as promising cell candidates to regenerate damaged hearts, because of the potential in differentiating to cardiomyocytes. However, the differentiation is difficult to trigger without inducers. Here we reported that β-cyclodextrin (β-CD) increased the expression of cardiac transcription factors (Nkx2.5 and GATA4), structural proteins (cardiac Troponin T, cTnt), transcriptional enhancer (Mef2c) and induced GATA4 nucleus translocation in adult resident CSCs, thus β-CD could be used to enhance myogenic transition. As the differentiation process was accompanied by autophagy, we constructed the Atg5 knockdown cell line by using the Atg5 siRNA lentivirus, and the myogenic conversion was blocked in Atg5 knockdown cells, which suggested that β-CD induces the cardiomyocytes transition of resident CSCs through autophagy. Furthermore, we found that JNK/STAT3 and GSK3β/β-catenin was the downstream pathways of β-CD-induced autophagy and differentiation using the inhibitors. Moreover, β-CD performed its functions through improving intracellular cholesterol levels and affecting cholesterol efflux. Collectively, our results reveal that β-CD as a novel tool to induce myogenic transition of CSCs, which could...Continue Reading

Citations

Feb 7, 2019·Journal of Sports Sciences·Kelly de JesusRicardo Jorge Pinto Fernandes
Nov 21, 2019·International Journal of Molecular Medicine·Ziang YaoHaige Wu
Oct 3, 2020·Pharmaceuticals·Adrián MatencioFrancesco Trotta
May 13, 2019·ACS Biomaterials Science & Engineering·Jianghong HuangDaping Wang

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