β-defensin 2 as an adjuvant promotes anti-melanoma immune responses and inhibits the growth of implanted murine melanoma in vivo.

PloS One
Han-fang MeiJuan Shen

Abstract

β-defensin 2 is a small antimicrobial peptide of the innate immune system and has been thought to regulate anti-tumor immunity. However, little is known on whether β-defensin 2 could modulate melanoma-specific NK and T cell responses. In this study, we first cloned the murine β-defensin 2 gene by RT-PCR and generated the β-defensin 2 stably expressing B16 cells (B16-mBD2). Subsequently, we evaluated whether vaccination with irradiated B16-mBD2 could modulate the growth of implanted B16 cells and determined the potential mechanisms underlying the action of B16-mBD2 vaccine in modulating the growth of B16 tumors in C57BL/6. We found that vaccination with irradiated B16-mBD2, but not with control B16-p or parental B16, inhibited the development and progression of B16 tumors, and prolonged the survival of tumor-bearing mice. However, vaccination with irradiated B16-mBD2 failed to inhibit the development of B16 tumors in the CD4(+)- or CD8(+)-depleted recipients. Furthermore, vaccination with irradiated B16-mBD2 stimulated strong NK activity and promoted potent B16-specific CTL responses, accompanied by augmenting IFN-γ and IL-12, but not IL-4, responses in the recipient mice. Moreover, vaccination with irradiated B16-mBD2 promoted ...Continue Reading

References

Jul 7, 1997·The Journal of Experimental Medicine·S R BennettW R Heath
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A BiragynL W Kwak
Aug 26, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Dajing XiaXuetao Cao
Jun 7, 2005·Expert Opinion on Emerging Drugs·Jean-Claude Bystryn, Jennifer L Rudolph
Jul 2, 2005·Expert Opinion on Emerging Drugs·Jean-Claude Bystryn
Nov 17, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yong-sheng WangYu-quan Wei
Feb 12, 2008·Investigative Ophthalmology & Visual Science·Hiroshi MochimaruSusumu Ishida
Feb 26, 2008·Expert Opinion on Biological Therapy·Marko Lens
Aug 30, 2008·Biochemical and Biophysical Research Communications·Wei ZhangYuquan Wei
Jun 18, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Natalia LaptevaXue F Huang

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Citations

Jun 13, 2013·Human Immunology·Justyna JarczakEmilia Bagnicka
Nov 14, 2013·Physiological Genomics·K G MeadeC O'Farrelly
Feb 15, 2019·Frontiers in Immunology·Kieran G Meade, Cliona O'Farrelly
Jul 9, 2021·Frontiers in Molecular Biosciences·Santosh K Ghosh, Aaron Weinberg

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Methods Mentioned

BETA
PCR
flow cytometry
Assay
density gradient centrifugation
transfection
ELISA

Software Mentioned

CellQuest

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