β-Defensin-4 (HBD-4) is expressed in chondrocytes derived from normal and osteoarthritic cartilage encapsulated in PEGDA scaffold

Acta histochemica
Giuseppe MusumeciC Loreto

Abstract

Defensins are antibiotic peptides involved in host defense mechanisms, wound healing and tissue repair. Furthermore, they seem to play an important role in protection mechanisms in articular joints. The aim of this study was to investigate β-defensin-4 expression in chondrocytes taken from articular cartilage of knees of patients with osteoarthritis (OA) compared to normal cartilage, in vivo in explanted tissue, and in vitro in chondrocytes encapsulated in construct PEGDA hydrogels. The present investigation was conducted to try and elucidate the possible use of β-defensin-4 as a relevant marker for the eventual use of successive scaffold allografts, and to provide new insights for hydrogel PEGDA scaffold efficacy in re-differentiation or repair of OA chondrocytes in vitro. Articular cartilage specimens from OA cartilage and normal cartilage were assessed by histology, histochemistry, immunohistochemistry and Western blot analysis. The results showed strong β-defensin-4 immunoexpression in explanted tissue from OA cartilage and weak β-defensin-4 expression in control cartilage. The chondrocytes from OA cartilage after 4 weeks of culture in PEGDA hydrogels showed the formation of new hyaline cartilage and a decreased expression ...Continue Reading

References

Jan 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J A van der SluijsJ Drukker
Jun 1, 1995·Bio/technology·J A Hubbell
May 29, 2000·Journal of Biomedical Materials Research·J ElisseeffR Langer
Oct 3, 2000·Arthritis and Rheumatism·M B Goldring
Aug 14, 2001·The Anatomical Record·A GutowskaM Jasionowski
Aug 31, 2001·Clinical and Diagnostic Laboratory Immunology·F PaulsenB Tillmann
Jan 25, 2002·Nature·Michael Zasloff
Oct 11, 2002·The Journal of Pathology·Friedrich PaulsenWolf Petersen
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Koichi MasudaEugene J-M A Thonar
Nov 6, 2004·Arthritis and Rheumatism·Deike VarogaFriedrich P Paulsen
Sep 9, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Peter S BurrageConstance E Brinckerhoff
Oct 26, 2005·Osteoarthritis and Cartilage·K P H PritzkerW B van den Berg
Mar 25, 2006·Biomaterials·Yuan JiMiriam H Rafailovich
Jun 9, 2006·Progress in Histochemistry and Cytochemistry·Helga Lorenz, Wiltrud Richter
Jul 21, 2010·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Giuseppe MusumeciGiuseppa Martinez

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Citations

Mar 5, 2013·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Edoardo SicurezzaRosalia Leonardi
Aug 1, 2013·International Journal of Molecular Sciences·Giuseppe MusumeciAnnelie Martina Weinberg
Jun 12, 2014·In Vitro Cellular & Developmental Biology. Animal·Jiaming Xu, Changqing Zhang
Apr 23, 2013·Acta histochemica·Venera CardileGiuseppe Musumeci
Oct 12, 2013·Neurobiology of Aging·Daniela PuzzoAgostino Palmeri
May 16, 2014·World Journal of Orthopedics·Giuseppe MusumeciSergio Castorina
Jan 1, 2014·F1000Research·Giuseppe MusumeciPaola Castrogiovanni
Jan 1, 2014·F1000Research·Giuseppe MusumeciPaola Castrogiovanni
Feb 8, 2019·Current Pharmaceutical Biotechnology·Marta A SzychlinskaGiuseppe Musumeci
May 7, 2020·Frontiers in Bioengineering and Biotechnology·Carla MouraNuno Alves
Aug 1, 2019·World Journal of Orthopedics·Silvia RavalliGiuseppe Musumeci
Sep 26, 2020·Frontiers in Physiology·Yue ZhaiYuanyuan Wang
Feb 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ximiao ChenChaoyong Bei
Feb 13, 2021·Journal of Functional Morphology and Kinesiology·Grazia Maugeri, Giuseppe Musumeci
Mar 31, 2021·Osteoarthritis and Cartilage·Liang ChangDongquan Shi

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