λ-DNA- and Aptamer-Mediated Sorting and Analysis of Extracellular Vesicles

Journal of the American Chemical Society
Chao LiuJiashu Sun

Abstract

Extracellular vesicles (EVs) are heavily implicated in diverse pathological processes. Due to their small size, distinct biogenesis, and heterogeneous marker expression, isolation and detection of single EV subpopulations are difficult. Here, we develop a λ-DNA- and aptamer-mediated approach allowing for simultaneous size-selective separation and surface protein analysis of individual EVs. Using a machine learning algorithm to EV signature based on their size and marker expression, we demonstrate that the isolated microvesicles are more efficient than exosomes and apoptotic bodies in discriminating breast cell lines and Stage II breast cancer patients with varied immunohistochemical expression of HER2. Our method provides an important tool to assess the EV heterogeneity at the single EV level with potential value in clinical diagnostics.

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Citations

Nov 14, 2019·Lab on a Chip·Tianlong ZhangMing Li
Jan 29, 2020·Journal of Cachexia, Sarcopenia and Muscle·Shuang RongWei Yang
Oct 9, 2020·Chemistry, an Asian Journal·Zejun JiangJianping Li
Feb 27, 2021·Analytical Chemistry·He YanYong Zeng
Feb 26, 2020·Biosensors & Bioelectronics·Su WangZhiyang Li
Nov 11, 2020·ACS Applied Materials & Interfaces·Jing XueYongxi Zhao
Mar 6, 2021·Chemical Reviews·Lingling WuWeihong Tan
Mar 28, 2021·Mikrochimica Acta·Shiana MalhotraRenee V Goreham
Jun 3, 2021·International Journal of Molecular Sciences·Raphael Rodrigues CorrêaRafael Soares Lindoso
May 21, 2021·Journal of Extracellular Vesicles·Yaxuan LiangMengrou Lu
Jun 3, 2021·International Journal of Molecular Sciences·Dhruvajyoti RoyJudith C Sporn
Jun 24, 2021·ACS Applied Materials & Interfaces·Huidong HuangWeihong Tan
Apr 20, 2021·Annual Review of Biomedical Engineering·Lia A StanciuNoor Mohammad
Apr 27, 2021·Annual Review of Analytical Chemistry·Chao Liu, Jiashu Sun
Apr 7, 2020·Analytical Chemistry·Xue-Nan FengDe-Ming Kong
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich
Jan 28, 2020·Journal of the American Chemical Society·Feng ChenYongxi Zhao
Oct 28, 2019·Analytical Chemistry·Erin Marie McConnellYingfu Li

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