* Efficacy of a Self-Assembling Peptide Hydrogel, SPG-178-Gel, for Bone Regeneration and Three-Dimensional Osteogenic Induction of Dental Pulp Stem Cells

Tissue Engineering. Part a
Jun TsukamotoTatsuaki Matsubara

Abstract

The aim of this study was to assess the efficacy of a self-assembling peptide hydrogel as a scaffold for bone regeneration. We used a neutral and injectable self-assembling peptide hydrogel, SPG-178-Gel. Bone defects (5 mm in diameter) in rat calvarial bones were filled with a mixture of alpha-modified Eagle's medium and peptide hydrogel. Three weeks after surgery, soft X-ray and microcomputed tomography (micro-CT) images of the gel-treated bones showed new bone formations in the periphery and in central areas of the defects. Next, we evaluated the three-dimensional osteogenic induction of dental pulp stem cells (DPSCs), a type of mesenchymal stem cell, in SPG-178-Gel. We first confirmed that the osteogenic differentiation of DPSCs was significantly promoted by osteogenic induction medium containing recombinant human bone morphogenetic protein-4 (rhBMP-4) in a two-dimensional cell culture. Then, we verified DPSC proliferation and osteogenic differentiation in a three-dimensional cell culture using SPG-178-Gel. The gene expression levels of osteopontin, osteocalcin, and collagen type I were significantly increased when DPSCs were cultured in SPG-178-Gel with the osteogenic induction medium. Micro-CT observations showed the forma...Continue Reading

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Citations

Jul 29, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kei AndoNaoki Ishiguro
Jan 10, 2019·Journal of Clinical Periodontology·Mark BartoldSaso Ivanovski
Sep 16, 2017·Journal of Biomedical Materials Research. Part a·Jean De La Croix NdongSteven Goudy
Sep 6, 2019·MRS Communications·Catherine S Millar-HaskellJason P Gleghorn
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Nov 21, 2020·Chemical Reviews·Febrizio GelainShuguang Zhang
Dec 8, 2021·Journal of Biomedical Materials Research. Part a·Hongchuan TianZhongliang Deng

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Methods Mentioned

BETA
X-ray
fluorescence microscopy
PCR

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