β-elemene alleviates airway stenosis via the ILK/Akt pathway modulated by MIR143HG sponging miR-1275.

Cellular & Molecular Biology Letters
Guoying ZhangYi-Ming Zeng

Abstract

We have previously found that β-elemene could inhibit the viability of airway granulation fibroblasts and prevent airway hyperplastic stenosis. This study aimed to elucidate the underlying mechanism and protective efficacy of β-elemene in vitro and in vivo. Microarray and bioinformatic analysis were used to identify altered pathways related to cell viability in a β-elemene-treated primary cell model and to construct a β-elemene-altered ceRNA network modulating the target pathway. Loss of function and gain of function approaches were performed to examine the role of the ceRNA axis in β-elemene's regulation of the target pathway and cell viability. Additionally, in a β-elemene-treated rabbit model of airway stenosis, endoscopic and histological examinations were used to evaluate its therapeutic efficacy and further verify its mechanism of action. The hyperactive ILK/Akt pathway and dysregulated LncRNA-MIR143HG, which acted as a miR-1275 ceRNA to modulate ILK expression, were suppressed in β-elemene-treated airway granulation fibroblasts; β-elemene suppressed the ILK/Akt pathway via the MIR143HG/miR-1275/ILK axis. Additionally, the cell cycle and apoptotic phenotypes of granulation fibroblasts were altered, consistent with ILK/Akt...Continue Reading

References

Oct 24, 1998·The International Journal of Biochemistry & Cell Biology·D G Greenhalgh
Apr 6, 1999·International Journal of Pediatric Otorhinolaryngology·J E DoharP A Hebda
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Feb 15, 2001·Annals of Internal Medicine·M SelmanUNKNOWN American College of Chest Physicians
Oct 2, 2002·Journal of Vascular and Interventional Radiology : JVIR·Kwang-Hun LeeWoo Sung Kim
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yining ZhangYasushi Matsuzaki
Jul 3, 2007·Cell·Brendan D Manning, Lewis C Cantley
Dec 17, 2008·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Giovanni GalluccioRaffaele Dello Iacono
Feb 4, 2009·Nature Reviews. Genetics·Tim R MercerJohn S Mattick
Jun 23, 2009·Journal of the American Society of Nephrology : JASN·Yingjian LiYouhua Liu
Jan 19, 2010·Journal of Cardiothoracic Surgery·Nader Abdel RahmanMordechai R Kramer
Sep 22, 2010·Virchows Archiv : an International Journal of Pathology·Panagiotis KavvadasAristidis S Charonis
Aug 2, 2011·Cell·Leonardo SalmenaPier Paolo Pandolfi
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Aug 21, 2013·Journal of Dermatological Science·Nicholas S GreavesArdeshir Bayat
Dec 4, 2013·Thoracic Surgery Clinics·Lutz Freitag, Kaid Darwiche
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
Apr 8, 2014·Archivos de bronconeumología·David Barros CasasAdnan Majid
Apr 19, 2015·Journal of Hematology & Oncology·Avencia Sanchez-Mejias, Yvonne Tay
Oct 7, 2015·Trends in Cell Biology·Hiro-Oki Iwakawa, Yukihide Tomari
Apr 5, 2016·Nature Reviews. Genetics·Daniel W Thomson, Marcel E Dinger
Nov 4, 2016·Nature Reviews. Drug Discovery·Masayuki Matsui, David R Corey
Oct 27, 2016·Respiration; International Review of Thoracic Diseases·Lingling HongDongyong Yang
May 5, 2017·Nucleic Acids Research·Mulin Jun LiX Shirley Liu
Nov 25, 2017·Nature Reviews. Cancer·Eleni AnastasiadouFrank J Slack
Jul 20, 2018·Journal of Cellular and Molecular Medicine·Dong-Mei WuYuan-Lin Zheng
Aug 7, 2018·Biochemical and Biophysical Research Communications·Jianwei ZhouRaoying Xie
Nov 14, 2018·Nucleic Acids Research·Ana KozomaraSam Griffiths-Jones
Jan 6, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Elwira SieniawskaKrystyna Skalicka-Woźniak

❮ Previous
Next ❯

Software Mentioned

Agilent Feature Extraction
ggplot2
GeneSpring GX
FlowJo
Arraystar
KEGG mapper
ImageJ
GraphPad Prism
miRbase

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis