β-elemene enhances anticancer bone neoplasms efficacy of paclitaxel through regulation of GPR124 in bone neoplasms cells

Oncology Letters
Zongze WangJian Hao

Abstract

The purpose of the present study was to investigate the anticancer effects of β-elemene and paclitaxel for bone neoplasms. MTT assay, reverse transcription-quantitative polymerase chain reaction, western blotting, flow cytometry and immunostaining were used to analyze the combined effects of β-elemene and paclitaxel both in vitro and in vivo. The results demonstrated that combined treatment of β-elemene and paclitaxel (β-elemene-paclitaxel) significantly inhibited growth and aggressiveness of U-2OS cells compared with either β-elemene or paclitaxel treatment alone. It was demonstrated that β-elemene promoted paclitaxel-induced apoptosis of U-2OS cells. Anti-apoptosis B-cell lymphoma (Bcl)-2 and Bcl-w genes were downregulated and pro-apoptotic Bcl-2-associated agonist of cell death and caspase-3 genes were upregulated in U-2OS cells following treatment with β-elemene-paclitaxel. Treatment of β-elemene-paclitaxel arrested the cell cycle and decreased cyclin-dependent kinase, cyclin-B1, P21 and P27 expression levels and decreased resistant genes alterations of ATP binding cassette subfamily B member 1, LDL receptor related protein and TS in U-2OS cells. Results demonstrated that β-elemene-paclitaxel decreased G-protein coupled rec...Continue Reading

References

Jul 3, 2010·Therapeutics and Clinical Risk Management·Leo KagerStefan Bielack
Jul 29, 2010·The Journal of Pharmacy and Pharmacology·Qingdi Quentin LiEddie Reed
Dec 14, 2011·The Journal of Pharmacy and Pharmacology·Jing LiuYun-Peng Liu
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Oct 27, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Çağrı ŞakalarHalit Canatan

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Citations

Apr 8, 2020·ACS Pharmacology & Translational Science·Abanoub A Gad, Nariman Balenga

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