Abstract
β-elemene (β-ELE) injection is a new anticancer drug extracted from Curcuma zedoaria Roscoe that has been widely used to treat malignant tumors. Recent studies show that β-ELE reverses the drug resistance of tumor cells. To explore the possible mechanisms of β-ELE, we investigated its effects on cisplatin (DDP)-resistant human lung adenocarcinoma A549/DDP cells. The effects of β-ELE on the growth of A549/DDP cells in vitro were determined by MTT assay. Apoptosis was assessed by fluorescence microscopy with Hoechst 33258 staining, flow cytometry with Annexin V-FITC/propium iodide double staining; mitochondrial membrane potential using JC-1 fluorescence probe and laser confocal scanning microscopy, and intracellular reactive oxygen species levels were measured by 2',7'-dichlorfluorescein-diacetate staining and flow cytometry; and contents of cytosolic glutathione were determined by glutathione assay kits. Intracellular Rhodamine-123 fluorescence intensity was detected by flow cytometry, and the expression of P-glycoprotein (P-gp) was detected by Western blotting. β-ELE inhibited the proliferation of A549/DDP cells in a time- and dose-dependent manner. Furthermore, β-ELE enhanced the sensitivity of A549/DDP cells to cisplatin and ...Continue Reading
References
Mar 2, 1999·Biochemical and Biophysical Research Communications·T NakamuraS Kohno
May 29, 2007·Oncogene·T E Stinchcombe, M A Socinski
Dec 19, 2007·Molecular Carcinogenesis·Jun HongStephen Safe
Feb 9, 2008·Annual Review of Plant Biology·Ruth FinkelsteinCamille Steber
Apr 3, 2008·Biochimica Et Biophysica Acta·Fei YanGui-Min Luo
Sep 17, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Jonathan F Tait
Apr 21, 2010·The Journal of Biological Chemistry·Susanne U MiedlichMarie B Demay
Feb 8, 2011·Journal of Cancer Research and Clinical Oncology·Zhen Ye XuXiao Yue Cai
Mar 1, 2012·Cancer Chemotherapy and Pharmacology·Fa-Jun XieYi-Ping Zhang
Jun 27, 2012·Biochimica Et Biophysica Acta·Magdalena L Circu, Tak Yee Aw
Jan 2, 2013·Medical Oncology·Qingdi Quentin LiEddie Reed
May 15, 2013·International Journal of Molecular Sciences·Ye ZhangYun-Peng Liu
Jun 12, 2013·Marine Drugs·Lili YangXiangyang Zou
Jul 3, 2013·International Journal of Oncology·Quentin Q LiEddie Reed
Jul 16, 2013·Toxicology Letters·A ProsperiniM J Ruiz
Aug 21, 2013·Gene·Li Zuo, Michael S Motherwell
Sep 28, 2013·The Oncologist·Sarah B GoldbergLecia V Sequist
Nov 29, 2013·Oncology Reports·Hui-Qin GuoZhe-Sheng Chen
Dec 18, 2013·Clinical Lung Cancer·Youngjoo LeeJin Soo Lee
Citations
Aug 8, 2019·Nutrition and Cancer·Hongxuan TongJingshang Wang
Aug 22, 2018·Oncology Letters·Lan LinTingyan Lin
Mar 7, 2020·Journal of Applied Physiology·Said H AudiElizabeth R Jacobs
Dec 6, 2018·Cell Proliferation·Ping ZhangLiming Tao
Jun 20, 2019·Pharmaceutical Research·Mingxiang CaoZhongping Chen
Feb 28, 2020·Pharmaceutics·Kambere Amerigos Daddy J CQineng Ping
Mar 4, 2020·International Journal of Molecular Medicine·Wencui KongZongyang Yu
Mar 10, 2020·BioMed Research International·Qiang XieChundong Gu
Nov 13, 2020·Cell Death & Disease·Shuiping LiuTian Xie
Mar 16, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yi ChenWan Liao
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ziqiang BaiTian Xie
May 25, 2021·Frontiers in Oncology·Hu CaiYongjun Zhang
Jun 8, 2021·Nutrition Research and Practice·Ki Mo KimSungwook Chae
Apr 10, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bingtao ZhaiXinbing Sui
Oct 14, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J WeiY Ding