β-Eudesmol induces JNK-dependent apoptosis through the mitochondrial pathway in HL60 cells

Phytotherapy Research : PTR
Yanchun LiEnlong Ma

Abstract

β-eudesmol, a natural sesquiterpenol present in a variety of Chinese herbs, is known to inhibit the proliferation of human tumor cells. However, the molecular mechanisms of the effect of β-eudesmol on human tumor cells are unknown. In the present study, we report the cytotoxic effect of β-eudesmol on the human leukemia HL60 cells and its molecular mechanisms. The cytotoxic effect of β-eudesmol on HL60 cells was associated with apoptosis, which was characterized by the presence of DNA fragmentation. β-eudesmol-induced apoptosis was accompanied by cleavage of caspase-3, caspase-9, and poly (ADP-ribose) polymerase; downregulation of Bcl-2 expression; release of cytochrome c from mitochondria; and decrease in mitochondrial membrane potential (MMP). Activation of c-Jun N-terminal kinases (JNK) mitogen-activated protein kinases was observed in β-eudesmol-treated HL60 cells, and the inhibitor of JNK blocked the β-eudesmol-induced apoptosis, downregulation of Bcl-2, and the loss of MMP. These data suggest that β-eudesmol induces apoptosis in HL60 cells via the mitochondrial apoptotic pathway, which is controlled through JNK signaling.

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Citations

Jun 22, 2013·Basic & Clinical Pharmacology & Toxicology·Diogo S BomfimDaniel P Bezerra
Sep 16, 2017·Journal of Complementary & Integrative Medicine·Wissem Aidi WannesBrahim Marzouk
Nov 20, 2016·Clinical and Experimental Pharmacology & Physiology·Na-Ra HanHyun-Ja Jeong
Jun 28, 2016·Molecular Biology Reports·Balraj Singh Gill Navgeet
Jun 20, 2018·BMC Pharmacology & Toxicology·Pimradasiri SrijiwangsaKesara Na-Bangchang
Jan 17, 2020·Chemistry & Biodiversity·Maria BailenAzucena Gonzalez-Coloma
Jan 16, 2021·Chemical Biology & Drug Design·Bishwanath AcharyaKesara Na-Bangchang

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