β-Glucanase Activity of the Oral Bacterium Tannerella forsythia Contributes to the Growth of a Partner Species, Fusobacterium nucleatum, in Cobiofilms

Applied and Environmental Microbiology
Kiyonobu HonmaAshu Sharma

Abstract

Tannerella forsythia and Fusobacterium nucleatum are dental plaque bacteria implicated in the development of periodontitis. These two species have been shown to form synergistic biofilms and have been found to be closely associated in dental plaque biofilms. A number of genetic loci for TonB-dependent membrane receptors (TDR) for glycan acquisition, with many existing in association with genes coding for enzymes involved in the breakdown of complex glycans, have been identified in T. forsythia In this study, we focused on a locus, BFO_0186-BFO_0188, that codes for a predicted TDR-SusD transporter along with a putative β-glucan hydrolyzing enzyme (BFO_0186). This operon is located immediately downstream of a 2-gene operon that codes for a putative stress-responsive extracytoplasmic function (ECF) sigma factor and an anti-sigma factor. Here, we show that BFO_0186 expresses a β-glucanase that cleaves glucans with β-1,6 and β-1,3 linkages. Furthermore, the BFO_0186-BFO_0188 locus is upregulated, with an induction of β-glucanase activity, in cobiofilms of T. forsythia and F. nucleatum The β-glucanase activity in mixed biofilms in turn leads to an enhanced hydrolysis of β-glucans and release of glucose monomers and oligomers as nutri...Continue Reading

References

Sep 1, 1986·Infection and Immunity·D Grenier, D Mayrand
Apr 1, 1995·Infection and Immunity·H M FletcherF L Macrina
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Jan 11, 2001·Biochimica Et Biophysica Acta·A Planas
Jan 7, 2004·Journal of Microbiological Methods·T ThurnheerB Guggenheim
Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paul G EglandPaul E Kolenbrander
Dec 23, 2004·Oral Microbiology and Immunology·A SharmaH K Kuramitsu
Jan 28, 2006·Cell·Ravichandran RamasamyAnn Marie Schmidt
Mar 17, 2007·Microbial Pathogenesis·Kiyonobu HonmaAshu Sharma
Aug 21, 2007·Bioresource Technology·Runping HanLina Zou
Jun 26, 2009·The Journal of Biological Chemistry·Eric C MartensJeffrey I Gordon
Aug 12, 2009·Journal of Proteome Research·Paul D VeithEric C Reynolds
Dec 19, 2009·Periodontology 2000·Masae Kuboniwa, Richard J Lamont
Dec 26, 2009·Veterinary Immunology and Immunopathology·Eva SonckEric Cox
Mar 3, 2010·PloS One·Vincent ZijngeHermie J M Harmsen
Nov 17, 2010·Infection and Immunity·Kiyonobu HonmaAshu Sharma
Apr 13, 2011·International Journal of Oral Science·Paul E Kolenbrander
Apr 24, 2012·Molecular Oral Microbiology·S S YanamandraJ P Lewis
Apr 12, 2013·Periodontology 2000·Ricardo TelesAnne Haffajee
Nov 5, 2013·Methods in Enzymology·Marina Santiago, Scott Strobel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.