β-Hexachlorocyclohexane: A Small Molecule with a Big Impact on Human Cellular Biochemistry

Biomedicines
Elisabetta RubiniMargherita Eufemi

Abstract

Organochlorine pesticides (OCPs) belong to a heterogeneous class of organic compounds blacklisted by the Stockholm Convention in 2009 due to their harmful impact on human health. Among OCPs, β-hexachlorocyclohexane (β-HCH) is one of the most widespread and, at the same time, poorly studied environmental contaminant. Due to its physicochemical properties, β-HCH is the most hazardous of all HCH isomers; therefore, clarifying the mechanisms underlying its molecular action could provide further elements to draw the biochemical profile of this OCP. For this purpose, LNCaP and HepG2 cell lines were used as models and were subjected to immunoblot, immunofluorescence, and RT-qPCR analysis to follow the expression and mRNA levels, together with the distribution, of key biomolecules involved in the intracellular responses to β-HCH. In parallel, variations in redox homeostasis and cellular bioenergetic profile were monitored to have a complete overview of β-HCH effects. Obtained results strongly support the hypothesis that β-HCH could be an endocrine disrupting chemical as well as an activator of AhR signaling, promoting the establishment of an oxidative stress condition and a cellular metabolic shift toward aerobic glycolysis. In this al...Continue Reading

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Citations

Jun 3, 2021·International Journal of Molecular Sciences·Elisabetta RubiniMargherita Eufemi

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Methods Mentioned

BETA
electrophoresis
PCR
Flow
Assay
nuclear translocation

Software Mentioned

GraphPad Prisma
CFX ManagerTM Real Time PCR Detection System

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