α-Imaging Confirmed Efficient Targeting of CD45-Positive Cells After 211At-Radioimmunotherapy for Hematopoietic Cell Transplantation

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Sofia H L FrostB Sandmaier

Abstract

α-radioimmunotherapy targeting CD45 may substitute for total-body irradiation in hematopoietic cell transplantation (HCT) preparative regimens for lymphoma. Our goal was to optimize the anti-CD45 monoclonal antibody (mAb; CA12.10C12) protein dose for (211)At-radioimmunotherapy, extending the analysis to include intraorgan (211)At activity distribution and α-imaging-based small-scale dosimetry, along with immunohistochemical staining. Eight normal dogs were injected with either a 0.75 (n = 5) or 1.00 (n = 3) mg/kg dose of (211)At-B10-CA12.10C12 (11.5-27.6 MBq/kg). Two were euthanized and necropsied 19-22 h after injection, and 6 received autologous HCT 3 d after (211)At-radioimmunotherapy, after lymph node and bone marrow biopsies at 2-4 and/or 19 h after injection. Blood was sampled to study toxicity and clearance; CD45 targeting was evaluated by flow cytometry. (211)At localization and small-scale dosimetry were assessed using two α-imaging systems: an α-camera and an ionizing-radiation quantum imaging detector (iQID) camera. (211)At uptake was highest in the spleen (0.31-0.61% injected activity [%IA]/g), lymph nodes (0.02-0.16 %IA/g), liver (0.11-0.12 %IA/g), and marrow (0.06-0.08 %IA/g). Lymphocytes in blood and marrow were ...Continue Reading

References

Jan 1, 1973·Transplantation·R StorbE D Thomas
Apr 23, 2004·Leukemia & Lymphoma·Marc H DahlkeHans J Schlitt
Jul 30, 2008·Seminars in Nuclear Medicine·Amin I Kassis
Jan 19, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·George SgourosPat B Zanzonico
Sep 18, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Tom Bäck, Lars Jacobsson
Jun 14, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Nicolas ChouinTom Bäck
Dec 18, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Hortense A KirişliWiro J Niessen
Jan 8, 2014·International Journal of Medical Sciences·Jun LiLanjuan Li
Jul 15, 2015·Nuclear Instruments & Methods in Physics Research. Section A, Accelerators, Spectrometers, Detectors and Associated Equipment·Brian W MillerLars R Furenlid

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Citations

Apr 17, 2017·Trends in Pharmacological Sciences·Stephanie M Stanford, Nunzio Bottini
Mar 10, 2017·International Journal of Radiation Biology·Anissa TazrartNina M Griffiths
Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yotis A Senis, Alastair J Barr
Jun 9, 2016·Nuclear Medicine and Molecular Imaging·Jin Su Kim
May 10, 2020·Veterinary and Comparative Oncology·Scott S Graves, Rainer Storb
Mar 21, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frédéric BaronArnon Nagler
Jun 18, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Alexander N GarciaJoe Gn Garcia
Mar 24, 2020·Chemical Reviews·Weijun WeiWeibo Cai

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