γδ Intraepithelial Lymphocyte Migration Limits Transepithelial Pathogen Invasion and Systemic Disease in Mice

Gastroenterology
Karen L EdelblumJerrold R Turner

Abstract

Intraepithelial lymphocytes that express the γδ T-cell receptor (γδ IELs) limit pathogen translocation across the intestinal epithelium by unknown mechanisms. We investigated whether γδ IEL migration and interaction with epithelial cells promote mucosal barrier maintenance during enteric infection. Salmonella typhimurium or Toxoplasma gondii were administered to knockout (KO) mice lacking either the T cell receptor δ chain (Tcrd) or CD103, or control TcrdEGFP C57BL/6 reporter mice. Intravital microscopy was used to visualize migration of green fluorescent protein (GFP)-tagged γδ T cells within the small intestinal mucosa of mice infected with DsRed-labeled S typhimurium. Mixed bone marrow chimeras were generated to assess the effects of γδ IEL migration on early pathogen invasion and chronic systemic infection. Morphometric analyses of intravital video microscopy data showed that γδ IELs rapidly localized to and remained near epithelial cells in direct contact with bacteria. Within 1 hour, greater numbers of T gondii or S typhimurium were present within mucosae of mice with migration-defective occludin KO γδ T cells, compared with controls. Pathogen invasion in Tcrd KO mice was quantitatively similar to that in mice with occlud...Continue Reading

References

Nov 1, 1989·The Journal of Experimental Medicine·T Goodman, L Lefrancois
Feb 14, 1998·The Journal of Cell Biology·J M HigginsM B Brenner
Dec 5, 2000·Molecular Biology of the Cell·M SaitouS Tsukita
Oct 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yaping ChenRichard Boismenu
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyoko Inagaki-OharaAkihiko Yoshimura
Oct 2, 2004·The Journal of Biological Chemistry·Fatme Al-AnoutiSirinart Ananvoranich
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anisa S IsmailLora V Hooper
Mar 31, 2010·The Journal of Cell Biology·Amanda M MarchiandoJerrold R Turner
Oct 16, 2010·Science·C V SrikanthBeth A McCormick
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anisa S IsmailLora V Hooper
Apr 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen L EdelblumJerrold R Turner
Jun 27, 2012·Annals of the New York Academy of Sciences·Caroline M Weight, Simon R Carding
Jul 31, 2013·Immunity·Brian S SheridanLeo Lefrançois
Jan 7, 2014·Annual Review of Immunology·Yueh-hsiu ChienMarc Bonneville
Feb 8, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Olivia I KoberNathalie Juge
Apr 8, 2014·Current Opinion in Immunology·Judith N MandlRonald N Germain

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Citations

Jan 23, 2016·Frontiers in Cell and Developmental Biology·Olivia B ParksMisty Good
Mar 8, 2016·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Cynthia Reinoso WebbMatthew B Grisham
Aug 14, 2015·Current Opinion in Infectious Diseases·Michael S BennettDaniel T Leung
Nov 22, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Richard L WuJerrold R Turner
Jan 27, 2017·Annual Review of Immunology·Yosuke Kurashima, Hiroshi Kiyono
Apr 23, 2017·The Journal of Experimental Medicine·Ana M C FariaDaniel Mucida
Dec 16, 2017·Current Pharmacology Reports·Madeleine D Hu, Karen L Edelblum
Jun 10, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Madeleine D HuKaren L Edelblum
Jun 16, 2017·Microbiology and Molecular Biology Reviews : MMBR·Rémi GayetBlaise Corthésy
Oct 28, 2017·Frontiers in Immunology·Špela KonjarMarc Veldhoen
Aug 28, 2019·Immunological Medicine·Hayakazu Sumida
Feb 28, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ali NazmiDanyvid Olivares-Villagómez
Sep 25, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Su-Man KimHyung-Sik Kang
Jul 15, 2020·Nature Reviews. Rheumatology·Eric GraceyDirk Elewaut
Aug 28, 2020·Immunological Reviews·Matthew A FischerKaren L Edelblum
Apr 25, 2018·Frontiers in Immunology·Deborah A WitherdenWendy L Havran
Dec 13, 2018·Frontiers in Immunology·Camille KhairallahBrian S Sheridan
Jan 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Anneke WilharmAvi-Hai Hovav
Jul 10, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adrian C Hayday
Aug 11, 2020·Bioscience of Microbiota, Food and Health·Hayakazu Sumida
Jul 6, 2018·Nature Reviews. Gastroenterology & Hepatology·Lisanne LutterFemke van Wijk
Mar 30, 2018·Frontiers in Immunology·Gabrielle M Siegers
May 18, 2020·Mucosal Immunology·Maud VandereykenMahima Swamy
Nov 13, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christa ParkJulie M Jameson
Apr 30, 2017·Tissue Barriers·Emily J JonesSimon R Carding
Mar 21, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luc Van Kaer, Danyvid Olivares-Villagómez
Dec 12, 2018·Frontiers in Immunology·Yu Anne Yap, Eliana Mariño
Nov 7, 2019·Journal of Immunology Research·Li WangSong Qin
Jul 3, 2020·Frontiers in Immunology·Jayne E WiardaCrystal L Loving
Jan 26, 2021·Clinical & Translational Immunology·Yu Anne YapEliana Mariño
Oct 16, 2020·Immunological Reviews·Michele K Anderson, Johanna S Selvaratnam

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