β-Lactoglobulin (BLG) binding to highly charged cationic polymer-grafted magnetic nanoparticles: effect of ionic strength

Journal of Colloid and Interface Science
Li QinXuhong Guo

Abstract

Poly(2-(methacryloyloxy)ethyltrimethyl ammonium chloride) (PMATAC) modified magnetic nanoparticles (NPs) with a high zeta potential of ca. 50mV were synthesized by atom transfer radical polymerization (ATRP). The prepared NPs consist of a magnetic core around 13nm and a PMATAC shell around 20nm attached on the surface of magnetic nanoparticles. Thermodynamic binding parameters between β-lactoglobulin and these polycationic NPs were investigated at different ionic strengths by high-resolution turbidimetry, dynamic light scattering (DLS), and isothermal titration calorimetry (ITC). Both turbidity and ITC show that binding affinities for BLG display a non-monotonic ionic strength dependence trend and a maximum appears at ionic strength of 50mM. Such observation should arise from the coeffects of protein charge anisotropy visualized by DelPhi electrostatic modeling and the strong electrostatic repulsion among highly charged NPs at a variety of ionic strengths.

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Citations

Jul 6, 2016·Advances in Colloid and Interface Science·Yisheng XuYanchuan Guo
May 20, 2017·The Analyst·Manuel AhumadaEmilio I Alarcon
Jan 17, 2021·Journal of Chromatography. a·Linling Yu, Yan Sun
May 26, 2021·The Science of the Total Environment·Shushan WuKimberly A Gray
Aug 11, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xiao XuMatthias Ballauff
Jun 24, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaohan WangXuhong Guo
Aug 15, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Kai ZhengYisheng Xu

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