α-Melanocyte-stimulating hormone inhibits tumor necrosis factor α-stimulated MUC5AC expression in human nasal epithelial cells

American Journal of Respiratory Cell and Molecular Biology
Sang-Nam LeeJoo-Heon Yoon

Abstract

Mucin hypersecretion is an important clinical feature of several respiratory diseases, including asthma, cystic fibrosis, nasal allergy, rhinitis, and sinusitis. It has been shown that α-melanocyte-stimulating hormone (α-MSH), a proopiomelanocortin (POMC)-derived peptide, has immunomodulatory activities by inhibiting NF-κB activation induced by proinflammatory cytokines such as TNF-α. Because MUC5AC expression is known to be up-regulated by TNF-α via NF-κB activation, we evaluated the inhibitory effect of α-MSH on MUC5AC gene expression induced by TNF-α in normal human nasal epithelial (NHNE) cells. Melanocortin-1-receptor (MC-1R) was detected by RT-PCR, Western blotting, and immunofluorescent labeling in NHNE cells. α-MSH suppressed NF-κB/p65 phosphorylation induced by TNF-α as well as IkB-α degradation in a dose-dependent manner, as assessed by Western blotting. In addition, α-MSH inhibited TNF-α-induced nuclear translocation of NF-κB and NF-κB luciferase activity. Real-time quantitative PCR data showed that α-MSH inhibited TNF-α-induced expression of MUC5AC, and this effect of α-MSH was neutralized by knockdown of MC-1R using MC-1R shRNA lentivirus. Analyses using RT-PCR and Western blotting showed the expression of POMC and...Continue Reading

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Citations

Sep 2, 2014·Biological & Pharmaceutical Bulletin·Biswajit PodderYong-Sik Kim
Oct 9, 2014·Mediators of Inflammation·Ana Isabel MartínAsunción López-Calderón
Sep 2, 2014·Biochemical Pharmacology·Magdalena K KanevaStephen J Getting
Aug 24, 2016·Frontiers in Endocrinology·Anne MüllerHeike Biebermann
Aug 28, 2020·Frontiers in Pharmacology·Antonio RecchiutiMario Romano
Dec 20, 2011·The Journal of Allergy and Clinical Immunology·Markus BöhmUlrike Raap

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