κ-opioid receptor agonists may alleviate intestinal damage in cardiopulmonary bypass rats by inhibiting the NF-κB/HIF-1α pathway

Experimental and Therapeutic Medicine
Xiaoyan ZhangYugang Diao

Abstract

The aims of the present study were to investigate the protective effect of a κ-opioid receptor (KOR) agonist on intestinal barrier dysfunction in rats during cardiopulmonary bypass (CPB), as well as to examine the role of NF-κB and the transcription factor hypoxia-inducible factor-1α (HIF-1α) signaling pathway in the regulatory mechanism. A total of 50 rats were randomly divided into five groups, with 10 rats in each group: Sham surgery group (group Sham), CPB surgery group (group CPB), KOR agonist + CPB (group K), KOR agonist + specific KOR antagonist + CBP (group NK) and KOR agonist + NF-κB pathway specific inhibitor + CPB (group NF). Intestinal microcirculation was evaluated to determine intestinal barrier dysfunction in rats following CPB surgery. Hematoxylin and eosin (H&E) staining was used to observe intestinal tissue injury in the rats. ELISA was used to detect the inflammatory factors interleukin (IL)-1β, IL-6, IL10 and tumor necrosis factor-α, and the oxidative stress factors superoxidase dismutase, malondialdehyde and nitric oxide in serum. In addition, ELISA was used to investigate the serum levels of the intestinal damage markers D-lactic acid, diamine oxidase and intestinal fatty acid-binding protein. Western blot...Continue Reading

References

Jan 1, 1991·Journal of Sports Sciences·D CollinsI Davies
Jan 1, 1987·Scandinavian Journal of Gastroenterology. Supplement·T Hedner, J Cassuto
Apr 3, 1996·JAMA : the Journal of the American Medical Association·D W RiddingtonJ F Bion
Sep 25, 1998·Biology of Reproduction·Y Zhu, J E Pintar
Sep 25, 2003·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·Norimasa SawadaHideki Chiba
Jun 3, 2004·The Annals of Thoracic Surgery·Fabien DoguetJean Paul Bessou
Aug 3, 2007·Interactive Cardiovascular and Thoracic Surgery·Hans J GeisslerKhosro Hekmat
Sep 12, 2007·Critical Care : the Official Journal of the Critical Care Forum·Daniel De BackerCan Ince
Mar 21, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melanie L HartHolger K Eltzschig
Oct 16, 2008·World Journal of Gastroenterology : WJG·Ying-Jie SunJin Zhou
Mar 18, 2009·Arthritis Research & Therapy·Kathryn M OliverEoin P Cummins
Apr 7, 2009·Regulatory Peptides·Peter Holzer
Dec 24, 2010·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Leonid N MaslovStephen A Brown
Feb 2, 2011·Investigative Ophthalmology & Visual Science·Shahid HusainCraig E Crosson
May 7, 2011·World Journal of Gastroenterology : WJG·Ying-Jie SunTie-Zheng Zhang
Sep 19, 2012·Cell Cycle·Matthias KapplerAlexander W Eckert
Jan 25, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Yu Xue ZhaoBing Zhu
Jul 11, 2013·American Journal of Therapeutics·Lijun ZhangJianming Pei
Aug 7, 2013·Molecular Cancer·Bastian Hoesel, Johannes A Schmid
Oct 1, 2013·Journal of Pediatric Surgery·Bhani K Chawla, Daniel H Teitelbaum
Nov 19, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Songwei YangHua Yang
Jul 25, 2014·Nature Communications·Raquel Requejo-AguilarJuan P Bolaños
Jun 11, 2015·Best Practice & Research. Clinical Anaesthesiology·Stefan De Hert, Anneliese Moerman
Nov 1, 2015·Bulletin of Experimental Biology and Medicine·S Yu TsibulnikovYu B Lishmanov
May 5, 2016·Acta Cirúrgica Brasileira·Paulo Roberto Barbosa EvoraAlfredo José Rodrigues
Aug 5, 2016·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Ronald A Bronicki, Mark Hall
Dec 14, 2016·Heart, Lung & Circulation·Barbara AdamikWaldemar Gozdzik
Dec 17, 2016·Neuroscience Letters·Cecília Cerqueira Café-MendesTania Marcourakis
Jan 4, 2017·Burns : Journal of the International Society for Burn Injuries·Akinori OsukaMasashi Ueyama
Dec 1, 2017·General Thoracic and Cardiovascular Surgery·Yasutaka Hirata

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
ELISA
MDA

Software Mentioned

SPSS
Quantity One

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.