γ-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1

Applied and Environmental Microbiology
Daisuke KasaiMasao Fukuda

Abstract

The Rhodococcus jostii RHA1 gene cluster required for γ-resorcylate (GRA) catabolism was characterized. The cluster includes tsdA, tsdB, tsdC, tsdD, tsdR, tsdT, and tsdX, which encode GRA decarboxylase, resorcinol 4-hydroxylase, hydroxyquinol 1,2-dioxygenase, maleylacetate reductase, an IclR-type regulator, a major facilitator superfamily transporter, and a putative hydrolase, respectively. The tsdA gene conferred GRA decarboxylase activity on Escherichia coli. Purified TsdB oxidized NADH in the presence of resorcinol, suggesting that tsdB encodes a unique NADH-specific single-component resorcinol 4-hydroxylase. Mutations in either tsdA or tsdB resulted in growth deficiency on GRA. The tsdC and tsdD genes conferred hydroxyquinol 1,2-dioxygenase and maleylacetate reductase activities, respectively, on E. coli. Inactivation of tsdT significantly retarded the growth of RHA1 on GRA. The growth retardation was partially suppressed under acidic conditions, suggesting the involvement of tsdT in GRA uptake. Reverse transcription-PCR analysis revealed that the tsd genes constitute three transcriptional units, the tsdBADC and tsdTX operons and tsdR. Transcription of the tsdBADC and tsdTX operons was induced during growth on GRA. Inactiva...Continue Reading

References

May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
May 1, 1973·Journal of Medicinal Chemistry·D Düwel, H Metzger
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·S S PaoM H Saier
Feb 14, 2006·FEMS Microbiology Reviews·Antonio J Molina-HenaresJuan L Ramos
Sep 1, 1995·Applied and Environmental Microbiology·M SetoK Yano
May 10, 2006·Applied Microbiology and Biotechnology·Tsuyoshi MatsuiToru Nagasawa
Aug 31, 2006·Biochemistry·Aimin Liu, Hong Zhang
Sep 12, 2006·Applied and Environmental Microbiology·Yan HuangShuang-Jiang Liu
May 8, 2007·Applied Microbiology and Biotechnology·M VeselýM Pátek
Sep 18, 2007·Applied and Environmental Microbiology·Jonathan O SharpLisa Alvarez-Cohen
Apr 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hui ZhouYi Tang
Sep 1, 2009·Journal of Bacteriology·Daisuke KasaiEiji Masai
Nov 26, 2009·Applied and Environmental Microbiology·Yuki FukuharaEiji Masai
Mar 15, 2011·Journal of Bioscience and Bioengineering·Kenta YamamotoSeiji Negoro
Apr 6, 2011·Journal of Molecular Microbiology and Biotechnology·Naoto ArakiMasao Fukuda
Jun 26, 2012·Applied and Environmental Microbiology·Ying XuNing-Yi Zhou

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