α-Santalol, a derivative of sandalwood oil, induces apoptosis in human prostate cancer cells by causing caspase-3 activation

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Ajay BommareddyChandradhar Dwivedi

Abstract

The anticancer effects of α-santalol, a major component of sandalwood oil, have been reported against the development of certain cancers such as skin cancer both in vitro and in vivo. The primary objectives of the current study were to investigate the cancer preventive properties of α-santalol on human prostate cancer cells PC-3 (androgen independent and P-53 null) and LNCaP (androgen dependent and P-53 wild-type), and determine the possible mechanisms of its action. The effect of α-santalol on cell viability was determined by trypan blue dye exclusion assay. Apoptosis induction was confirmed by analysis of cytoplasmic histone-associated DNA fragmentation using both an apoptotic ELISA kit and a DAPI fluorescence assay. Caspase-3 activity was determined using caspase-3 (active) ELISA kit. PARP cleavage was analyzed using immunoblotting. α-Santalol at 25-75 μM decreased cell viability in both cell lines in a concentration and time dependent manner. Treatment of prostate cancer cells with α-santalol resulted in induction of apoptosis as evidenced by DNA fragmentation and nuclear staining of apoptotic cells by DAPI. α-Santalol treatment also resulted in activation of caspase-3 activity and PARP cleavage. The α-santalol-induced apop...Continue Reading

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Citations

Feb 19, 2013·DNA and Cell Biology·Somayeh VandghanooniJafar Ezzati Nazhad Dolatabadi
Jan 11, 2014·Organic & Biomolecular Chemistry·Pankaj P DaramwarHirekodathakallu V Thulasiram
May 16, 2015·Scientific Reports·Prabhakar Lal SrivastavaHirekodathakallu V Thulasiram
Jun 15, 2013·Journal of the Science of Food and Agriculture·Yashika BhallaVikas Jaitak
Feb 5, 2014·Food Chemistry·Morteza EskandaniJafar Ezzati Nazhad Dolatabadi
Feb 2, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Biswapriya B Misra, Satyahari Dey
Nov 14, 2017·Natural Product Research·Ajay BommareddyChandradhar Dwivedi
Dec 3, 2014·Chemical & Pharmaceutical Bulletin·Yukiko MatsuoYoshihiro Mimaki
Jan 3, 2021·Biochemical and Biophysical Research Communications·Sneha SinghKiran Kulkarni
Nov 6, 2021·Applied Microbiology and Biotechnology·Yuchen WangMengliang Wen

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