γ Sulphate PNA (PNA S): highly selective DNA binding molecule showing promising antigene activity.

PloS One
Concetta AvitabileAlessandra Romanelli

Abstract

Peptide Nucleic Acids (PNAs), nucleic acid analogues showing high stability to enzyme degradation and strong affinity and specificity of binding toward DNA and RNA are widely investigated as tools to interfere in gene expression. Several studies have been focused on PNA analogues with modifications on the backbone and bases in the attempt to overcome solubility, uptake and aggregation issues. γ PNAs, PNA derivatives having a substituent in the γ position of the backbone show interesting properties in terms of secondary structure and affinity of binding toward complementary nucleic acids. In this paper we illustrate our results obtained on new analogues, bearing a sulphate in the γ position of the backbone, developed to be more DNA-like in terms of polarity and charge. The synthesis of monomers and oligomers is described. NMR studies on the conformational properties of monomers and studies on the secondary structure of single strands and triplexes are reported. Furthermore the hybrid stability and the effect of mismatches on the stability have also been investigated. Finally, the ability of the new analogue to work as antigene, interfering with the transcription of the ErbB2 gene on a human cell line overexpressing ErbB2 (SKBR3)...Continue Reading

References

Nov 15, 1986·Analytical Biochemistry·R J GilliesM Denton
Dec 16, 1983·Biochemical and Biophysical Research Communications·M RanceK Wüthrich
Sep 1, 1996·Nucleic Acids Research·P J FinnT Brown
Jul 29, 1998·Journal of Peptide Science : an Official Publication of the European Peptide Society·G Aldrian-HerradaJ Brugidou
Jun 22, 1999·Chemistry & Biology·S E HamiltonD R Corey
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·B HerbertD R Corey
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
Feb 5, 2003·Advanced Drug Delivery Reviews·Uffe Koppelhus, Peter E Nielsen
Jun 6, 2003·Journal of the American Chemical Society·Thomas Bentin, Peter E Nielsen
Sep 18, 2003·Bioconjugate Chemistry·Andriy A Mokhir, Roland Kraemer
May 26, 2004·Biochemical and Biophysical Research Communications·Robert Roskoski
May 18, 2005·Accounts of Chemical Research·Vaijayanti A Kumar, Krishna N Ganesh
Nov 17, 2005·Journal of the American Chemical Society·Luigi PetracconeConcetta Giancola
Aug 3, 2006·Journal of the American Chemical Society·Anca Dragulescu-AndrasiDanith H Ly
Aug 21, 2007·Expert Opinion on Biological Therapy·Soccorsa PensatoAlessandra Romanelli
Nov 22, 2007·Journal of the American Chemical Society·Srinivas RapireddyDanith H Ly
Jan 22, 2008·Nucleic Acids Research·Takumi IshizukaMakoto Komiyama
Aug 12, 2009·Journal of the American Chemical Society·Gaofei HeDanith H Ly
Dec 22, 2009·Molecular BioSystems·Giovanni N RovielloCarlo Pedone
Jul 21, 2010·Bioorganic Chemistry·Soccorsa PensatoAlessandra Romanelli
Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joy ChenJames J Bieker
Jul 1, 1995·Journal of Biomolecular NMR·C BartelsK Wüthrich

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Citations

Aug 6, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria GaglioneAnna Messere
Jan 12, 2016·Artificial DNA, PNA & XNA·Maria MocciaMichele Saviano
Oct 24, 2015·Biophysical Chemistry·J M P ViévilleM A Delsuc
Jun 16, 2015·Bioconjugate Chemistry·Anjan BanerjeeVaijayanti A Kumar
Oct 16, 2015·PloS One·Yuliya KirillovaGalina Pozmogova
Jul 23, 2015·Beilstein Journal of Nanotechnology·Giovanni N RovielloMerab Mirtskhulava
Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Monika WojciechowskaJoanna Trylska
Oct 1, 2019·Chemistry : a European Journal·Concetta AvitabileAlessandra Romanelli
Feb 6, 2020·Chemical Communications : Chem Comm·Colin S Swenson, Jennifer M Heemstra
Dec 15, 2020·Chemical Biology & Drug Design·Anuradha Das, Biswaranjan Pradhan
Jun 16, 2021·Biopolymers·J Dinithi R PereraPeter M Glazer
Aug 10, 2021·Beilstein Journal of Organic Chemistry·Nikita BrodyaginEriks Rozners
Jan 5, 2019·The Journal of Organic Chemistry·Wei-Che HsiehDanith H Ly
Jun 17, 2015·Journal of the American Chemical Society·Iulia SacuiDanith H Ly

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Methods Mentioned

BETA
transfection
NMR
fluorescence microscopy
two hybrids
flow
FACS
Assay
fluorescence activated cell sorting

Software Mentioned

Cell Quest
CARA
VNMR

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