Abstract
α-Synuclein is a neuronal protein implicated in the etiology of Parkinson's disease (PD) and dementia with Lewy bodies (DLB). Whilst increased α-synuclein expression due to gene duplication or triplication can cause familial PD, previous studies of α-synuclein levels in idiopathic disease have produced conflicting data. We quantified α-synuclein mRNA and soluble protein in five human post-mortem brain regions from four groups of individuals with PD, DLB, Alzheimer's disease (AD) and matched controls. α-Synuclein mRNA levels, measured using quantitative real-time PCR, did not differ significantly between groups in any brain regions examined. In contrast, levels of soluble α-synuclein protein, measured by ELISA, were significantly lower in 4 of the 5 regions for patients with DLB, and in 2 of the 5 regions for patients with PD, compared to controls. Soluble α-synuclein protein levels were not significantly different in the AD patients, compared to controls, in 4 of the 5 regions. This study indicates that although levels of soluble α-synuclein protein are lower in DLB and PD, there is no evidence for a corresponding decrease in α-synuclein mRNA levels. This might result from altered translation, or removal of α-synuclein protein ...Continue Reading
References
Nov 24, 1995·Neuroscience Letters·P J HarrisonR C Pearson
Oct 8, 1999·Neuroreport·T A BayerK Beyreuther
Apr 29, 2000·Acta Neuropathologica·E Gómez-TortosaB T Hyman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 30, 2002·Journal of Molecular Endocrinology·S A Bustin
May 2, 2003·Journal of Neural Transmission·H BraakK Del Tredici
Oct 16, 2003·Brain Research. Molecular Brain Research·Paul Preece, Nigel J Cairns
Jan 22, 2004·Human Molecular Genetics·Jun Z LiRichard M Myers
Oct 21, 2005·Neurology·I G McKeithUNKNOWN Consortium on DLB
May 11, 2007·Acta Neurologica Scandinavica·Amos D Korczyn
Nov 7, 2007·Cell and Tissue Banking·Rivka Ravid
May 8, 2008·BMC Molecular Biology·David T R CoulsonJanet A Johnston
Jul 2, 2008·Acta Neuropathologica·Kurt A Jellinger
Feb 28, 2009·Clinical Chemistry·Stephen A BustinCarl T Wittwer
Sep 3, 2009·Nature Reviews. Neurology·Michael Eller, David R Williams
Jan 29, 2010·The Journal of Biological Chemistry·Epaminondas Doxakis
Dec 16, 2010·The European Journal of Neuroscience·James K R StevensonMartin J McKeown
Mar 18, 2011·Human Mutation·Eugénie MutezMarie-Christine Chartier-Harlin
Citations
Jan 15, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Vadim YuferovMary Jeanne Kreek
Jul 28, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Megan E LarsonSylvain E Lesné
Dec 25, 2012·International Journal of Molecular Sciences·Bidur BhandaryHan-Jung Chae
Mar 3, 2018·Frontiers in Molecular Neuroscience·Matthew J BenskeyFredric P Manfredsson
Feb 8, 2016·Journal of Neurochemistry·M J BenskeyF P Manfredsson
Mar 30, 2018·Neurochemical Research·Xiaohong WangDuonan Yu
Feb 15, 2018·Frontiers in Aging Neuroscience·Md Sahab UddinAdrian M Stankiewicz
Jun 20, 2019·Journal of Neural Transmission·Kurt A Jellinger
Sep 10, 2016·Molecular Brain·Huayang XingMitchell K P Lai
Apr 16, 2019·Frontiers in Neuroscience·H J van Heesbeen, Marten P Smidt
Mar 18, 2020·Acta Neuropsychiatrica·Anisa Chowdhury, Anto P Rajkumar