α-Synuclein mRNA and soluble α-synuclein protein levels in post-mortem brain from patients with Parkinson's disease, dementia with Lewy bodies, and Alzheimer's disease

Brain Research
Joseph G QuinnJanet A Johnston

Abstract

α-Synuclein is a neuronal protein implicated in the etiology of Parkinson's disease (PD) and dementia with Lewy bodies (DLB). Whilst increased α-synuclein expression due to gene duplication or triplication can cause familial PD, previous studies of α-synuclein levels in idiopathic disease have produced conflicting data. We quantified α-synuclein mRNA and soluble protein in five human post-mortem brain regions from four groups of individuals with PD, DLB, Alzheimer's disease (AD) and matched controls. α-Synuclein mRNA levels, measured using quantitative real-time PCR, did not differ significantly between groups in any brain regions examined. In contrast, levels of soluble α-synuclein protein, measured by ELISA, were significantly lower in 4 of the 5 regions for patients with DLB, and in 2 of the 5 regions for patients with PD, compared to controls. Soluble α-synuclein protein levels were not significantly different in the AD patients, compared to controls, in 4 of the 5 regions. This study indicates that although levels of soluble α-synuclein protein are lower in DLB and PD, there is no evidence for a corresponding decrease in α-synuclein mRNA levels. This might result from altered translation, or removal of α-synuclein protein ...Continue Reading

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Citations

Jan 15, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Vadim YuferovMary Jeanne Kreek
Jul 28, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Megan E LarsonSylvain E Lesné
Dec 25, 2012·International Journal of Molecular Sciences·Bidur BhandaryHan-Jung Chae
Mar 3, 2018·Frontiers in Molecular Neuroscience·Matthew J BenskeyFredric P Manfredsson
Feb 15, 2018·Frontiers in Aging Neuroscience·Md Sahab UddinAdrian M Stankiewicz
Apr 16, 2019·Frontiers in Neuroscience·H J van Heesbeen, Marten P Smidt
Mar 18, 2020·Acta Neuropsychiatrica·Anisa Chowdhury, Anto P Rajkumar

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