0.005% Preservative-Free Latanoprost Induces Dry Eye-Like Ocular Surface Damage via Promotion of Inflammation in Mice

Investigative Ophthalmology & Visual Science
Yiran YangZuguo Liu

Abstract

To investigate the side effects of preservative-free 0.005% latanoprost on the murine ocular surface. We applied 0.005% latanoprost or vehicle in mice in two patterns for 14 to 28 days. Tear production was measured by phenol red cotton test, and corneal epithelial barrier function was assessed by Oregon-green-dextran (OGD) staining. Periodic acid-Schiff (PAS) staining was used to quantify conjunctival goblet cells (GCs). The expression of matrix metalloproteinase (MMP)-3 and -9, occludin-1 and zonula occludens (ZO)-1 in corneal epithelium was assessed by immunofluorescent staining and/or quantitative real-time PCR (qRT-PCR). Inflammation in conjunctiva was assessed by activation of P38 and NF-κB, infiltration of CD4+ T cells, and production inflammatory cytokines including TNF-α, IL-1β, IFN-γ, IL-17A, and IL-13. Apoptosis in ocular surface was assessed by TUNEL and immunofluorescent staining for activated caspase-3 and -8. Cell viability assay was performed in human corneal epithelial cells. Topical latanoprost treatment decreased tear production, induced conjunctival GC loss, disrupted the corneal epithelial barrier, and promoted cell apoptosis in the ocular surface. Topical latanoprost treatment increased the expression of MM...Continue Reading

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Citations

Sep 11, 2019·Investigative Ophthalmology & Visual Science·Mingli QuQingjun Zhou
Aug 13, 2020·Drug Delivery and Translational Research·Xuefei LiXianggen Wu
Jun 18, 2020·Clinical Ophthalmology·Charles B TrelfordCindy M L Hutnik
Oct 20, 2020·Progress in Retinal and Eye Research·Christophe BaudouinElisabeth M Messmer

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