Abstract
1, 25-Dihydroxyvitamin D3 (1, 25(OH)2D3), an active form of vitamin D3, plays a crucial role in the mitigation of inflammation damage. Recent studies have revealed that apelin and its receptor (apelin/APJ system) could significantly ameliorate LPS-induced inflammation-response. This investigation aimed to appraise the effects of 1, 25(OH)2D3 on the apelin/APJ system and production of adhesion molecules and inflammatory mediators in LPS-activated RAW264.7 macrophage cells. Murine RAW264.7 cells were pretreated with 1, 25(OH)2D3, followed stimulation with LPS (1 μg/mL) for 24 h. The effect of 1, 25(OH)2D3 on LPS-induced cell injury was determined by MTT assay, whereas, enzyme-linked immunosorbent assay (ELISA), qPCR and western blotting were used to evaluate cytokine production and apelin/APJ system expression. Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) protein expression were measured by flow cytometry. The levels of IL-1β, IL-6, and TNF-α cytokines were significantly increased by incubation with LPS. LPS also increased the protein expression of adhesion molecules, including VCAM-1 and ICAM-1. However, pretreatment with 1, 25(OH)2D3 markedly inhibited LPS-induced production of infla...Continue Reading
References
May 5, 2001·Biochimica Et Biophysica Acta·Y KawamataM Fujino
May 9, 2001·The Journal of Infectious Diseases·G BaumgartenJ G Vallejo
Jul 11, 2006·The American Journal of Clinical Nutrition·Heike A Bischoff-FerrariBess Dawson-Hughes
Oct 20, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Maria StioAndrea G Bonanomi
Mar 24, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Nicholas J LeeperThomas Quertermous
Mar 27, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Amy E RiekCarlos Bernal-Mizrachi
Apr 30, 2010·Current Opinion in Pharmacology·Femke BaekeChantal Mathieu
Feb 15, 2011·Drug and Chemical Toxicology·Hichem SebaiNéziha Ghanem-Boughanmi
Jun 28, 2011·Applied Microbiology and Biotechnology·Ming LeiFengyang Wang
Jan 8, 2013·Atherosclerosis·Xiao-Yan LiuChao-Ke Tang
Jul 28, 2013·Immunobiology·Ioana Madalina Fenyo, Anca Violeta Gafencu
Sep 24, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xiao-Hua YuChao-Ke Tang
Mar 13, 2014·The Journal of Biological Chemistry·Qingsong WangYing Yu
Oct 4, 2014·Peptides·Qing XinBo Bai
Oct 15, 2014·Current Pharmaceutical Design·Alexander N OrekhovJulia Kzhyshkowska
Jan 13, 2015·Biochimie·Shan WuJianhua Qin
Apr 29, 2015·Nutrients·Margherita T CantornaLinlin Yang
May 13, 2015·Peptides·Fan YangYanrong Jiang
Nov 4, 2015·PloS One·Emily K CaltonMario J Soares
Dec 19, 2015·Physiological Reviews·Sylvia ChristakosGeert Carmeliet
Nov 20, 2016·Biochemical and Biophysical Research Communications·Dong MaJin-Kun Wen
Jan 31, 2017·Current Opinion in Lipidology·Mary M KavurmaDenuja Karunakaran
Mar 2, 2017·Physiological Research·V N Izgüt-UysalI Üstünel
Jun 28, 2017·Cell Biology International·Junfeng CuiYanling Jiang
Jan 1, 2018·Clinica Chimica Acta; International Journal of Clinical Chemistry·Diana Marcela GiraldoSilvio Urcuqui-Inchima
Jan 13, 2018·Cytokine·John GubatanAlan C Moss
Jun 23, 2018·Clinical Science·Glaucylara Reis Geovanini, Peter Libby
Jul 22, 2018·Biochemical and Biophysical Research Communications·Yousef FaridvandAhmadreza Jodati
Sep 21, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hailin ZhangXiaoxia Kong
Citations
Dec 18, 2019·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Saeed RadagdamSiavash Gerayesh Nejad
Feb 19, 2020·Expert Opinion on Therapeutic Patents·Conrad Fischer
Mar 7, 2020·Cells·Yu-Han WangChih-Hsin Tang
May 19, 2020·Aging·Ting-Kuo ChangChih-Hsin Tang
Feb 23, 2020·Cardiovascular Toxicology·Yousef FaridvandMohammad Nouri