Abstract
The Kaposi sarcoma-associated herpes virus-G protein-coupled receptor is a key molecule in the pathogenesis of Kaposi sarcoma, playing a central role in promoting vascular endothelial growth factor-driven angiogenesis and spindle cell proliferation. We studied the effects of 1 alpha,25-dihydroxyvitamin D(3) [1 alpha,25(OH)(2)D(3)] and the analog TX527 on the proliferation of endothelial cells (SVECs) and SVECs transformed by the viral G protein-coupled receptor (SVEC-vGPCR). 1 alpha,25(OH)(2)D(3) and TX527 decreased SVEC-vGPCR and SVEC numbers, the response being time dependent and similar in both cell lines. Vitamin D receptor (VDR) levels increased on treatment with 10 nm 1 alpha,25(OH)(2)D(3) or 1 nm TX527 in a time-dependent manner (1.5-24 h) in SVECs and SVEC-vGPCR. Basal VDR levels were increased in SVEC-vGPCR. The antiproliferative effects were accompanied by reduction in cyclin D1 and accumulation of p27 in SVECs but not SVEC-vGPCR. Induction of VDR was blocked by transfection of short hairpin RNA against VDR in SVEC-vGPCR and the antiproliferative effects of 1 alpha,25(OH)(2)D(3) and TX527 were decreased, involving the VDR genomic pathway in the hormone and analog mechanism of action. In vivo experiments showed that 1 ...Continue Reading
References
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·A W NormanM C Farach-Carson
Sep 23, 1995·Lancet·D WhitbyA S Denton
Oct 30, 1995·Molecular and Cellular Endocrinology·R BolandS Zanello
Dec 1, 1995·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·O M MemarS K Tyring
May 18, 1999·International Journal of Cancer. Journal International Du Cancer·K IsekiS Ishiguro
Feb 9, 2000·The Journal of Experimental Medicine·T Y YangS A Lira
Aug 6, 2000·Circulation Research·D J MantellA E Canfield
Sep 17, 2002·Urology·Candace S JohnsonDonald L Trump
Feb 1, 2003·Cancer Cell·Silvia MontanerJ Silvio Gutkind
Aug 16, 2003·Nutrition Reviews·Kathryn Z GuytonGary H Posner
May 6, 2004·Journal of Anxiety Disorders·Meredith E ColesRichard G Heimberg
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Paul Furigay, Narasimha Swamy
Jul 26, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Guy EelenAnnemieke Verstuyf
Aug 23, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Roger BouillonAnnemieke Verstuyf
Feb 18, 2006·The International Journal of Biochemistry & Cell Biology·Verónica González PardoAna Russo de Boland
Apr 11, 2006·The Journal of Clinical Investigation·Marcos G GrisottoSergio A Lira
Sep 5, 2006·Cancer Research·Ivy ChungDonald L Trump
Jan 26, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Lieve VerlindenAnnemieke Verstuyf
Apr 7, 2007·Cancer Letters·Wei WuLaura P Zanello
Apr 10, 2007·FEBS Letters·Elzbieta GocekEwa Marcinkowska
Jul 14, 2007·Current Medicinal Chemistry·G EelenA Verstuyf
Aug 28, 2007·Nature Reviews. Cancer·Kristin K DeebCandace S Johnson
Oct 16, 2007·Oncogene·D MartinJ S Gutkind
Jul 17, 2008·The Journal of Biological Chemistry·Jianrong WangSamantha Spindel
Jul 31, 2008·Cellular Signalling·Ruth M DenshamSimon J Cook
Aug 13, 2008·Endocrine Reviews·Roger BouillonMarie Demay
Dec 5, 2008·Nutrition Reviews·Hector F DeLuca
Citations
Jan 14, 2012·PloS One·Barbara KlotzFranz Jakob
Nov 20, 2012·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Guoxiang LaiYingfang Song
Nov 7, 2012·Scandinavian Journal of Immunology·Y SongG Lai
Apr 29, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Kristine M ErlandsonThomas B Campbell
Apr 29, 2014·Frontiers in Physiology·Carlien LeyssensAnnemieke Verstuyf
Aug 13, 2013·PloS One·Simona GurzuIoan Jung
Feb 6, 2017·Cellular Signalling·A SuaresV González-Pardo
Jul 28, 2012·Pulmonary Circulation·Harm J BogaardNorbert F Voelkel
Jan 5, 2011·Nature Reviews. Drug Discovery·Rosamaria Lappano, Marcello Maggiolini
Oct 12, 2017·Virologica Sinica·Lu DaiZhiqiang Qin
Apr 30, 2011·Obesity·Joram D MulEdwin Cuppen
Feb 22, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Andrzej T SlominskiCraig A Elmets
May 8, 2013·British Journal of Pharmacology·V González-PardoA Russo de Boland
Oct 1, 2017·Scientific Reports·Amit KumarTathagata Choudhuri
Oct 20, 2020·Heliyon·Cinthya TapiaVerónica González-Pardo