Abstract
1-Bromopropane (1-BP) has been used in industry as an alternative to ozone-depleting solvents. In the present study, we examined the effect of 1-BP on cyclooxygenase-2 (COX-2) gene expression and analyzed the molecular mechanism of its activity in murine RAW 264.7 macrophages. 1-BP dose-dependently increased COX-2 protein and mRNA levels, as well as COX-2 promoter-driven luciferase activity in macrophages. Additionally, exposure to 1-BP markedly enhanced the production of prostaglandin E(2) (PGE(2)), a major COX-2 metabolite, in macrophages. Transfection experiments with several human COX-2 promoter constructs revealed that 1-BP activated the transcription factors nuclear factor-κB (NF-κB) and CCAAT/enhancer-binding protein (C/EBP), but not AP-1 or the cyclic AMP response element binding protein. Furthermore, Akt and mitogen-activated protein (MAP) kinases were significantly activated by 1-BP. These results demonstrated that 1-BP induced COX-2 expression via NF-κB and C/EBP activation through the Akt/ERK and p38 MAP kinase pathways. These findings provide further insight into the signal transduction pathways involved in the inflammatory effects of 1-BP.
References
Nov 1, 1991·Cell·P Descombes, U Schibler
Oct 1, 1996·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·A J Whitmarsh, R J Davis
Feb 29, 2000·The Journal of Biological Chemistry·D J WadleighH R Herschman
Mar 21, 2000·Biochimica Et Biophysica Acta·S M Prescott, F A Fitzpatrick
Aug 31, 2000·Annual Review of Biochemistry·W L SmithR M Garavito
Feb 13, 2001·European Journal of Biochemistry·D Roymans, H Slegers
Mar 30, 2001·The Journal of Biological Chemistry·M A SaundersK K Wu
Jan 17, 2002·The Journal of Pathology·Bastiaan P van ReesG Johan A Offerhaus
Mar 21, 2002·The Lancet Oncology·P K Lala, C Chakraborty
May 22, 2002·Surgical Oncology·Buckminster Farrow, B Mark Evers
Jul 20, 2002·Molecular Pharmacology·Sarah A WardlawSteven A Belinsky
Aug 14, 2003·Journal of Molecular Endocrinology·M TamuraS E Bulun
Aug 19, 2003·Archives of Biochemistry and Biophysics·Hiroshi OhshimaTomohiro Sawa
Dec 10, 2003·Oncogene·Thomas F FrankeChizuru Sugimoto
Sep 18, 2004·Cancer Letters·Shan ZhaAngelo M De Marzo
Apr 2, 2005·Annales pharmaceutiques françaises·N Pommery, J-P Hénichart
Apr 7, 2005·International Archives of Occupational and Environmental Health·Gaku Ichihara
Apr 25, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Jennifer Juhl MajersikJohn D Steffens
Jan 1, 2008·Cancer Letters·Eun Hee HanHye Gwang Jeong
Oct 9, 2008·Toxicology and Applied Pharmacology·Eun Hee HanHye Gwang Jeong
Jul 27, 2010·Journal of Occupational and Environmental Medicine·Weihua LiGaku Ichihara
Citations
Jun 19, 2013·Archives of Pharmacal Research·Hyung Min JeongKwang Youl Lee
Oct 12, 2013·PloS One·Weiting QinBingwei Sun
Feb 11, 2015·Experimental and Therapeutic Medicine·Wei-Ting QinBing-Wei Sun
Dec 21, 2018·Neuroscience Bulletin·Lin XuXiu-Lan Zhao