1-FFT amino acids involved in high DP inulin accumulation in Viguiera discolor

Frontiers in Plant Science
Emerik De SadeleerWim Van den Ende

Abstract

Fructans are important vacuolar reserve carbohydrates with drought, cold, ROS and general abiotic stress mediating properties. They occur in 15% of all flowering plants and are believed to display health benefits as a prebiotic and dietary fiber. Fructans are synthesized by specific fructosyltransferases and classified based on the linkage type between fructosyl units. Inulins, one of these fructan types with β(2-1) linkages, are elongated by fructan:fructan 1-fructosyltransferases (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptor inulin molecule. The sequence identity of the 1-FFT of Viguiera discolor (Vd) and Helianthus tuberosus (Ht) is 91% although these enzymes produce distinct fructans. The Vd 1-FFT produces high degree of polymerization (DP) inulins by preferring the elongation of long chain inulins, in contrast to the Ht 1-FFT which prefers small molecules (DP3 or 4) as acceptor. Since higher DP inulins have interesting properties for industrial, food and medical applications, we report here on the influence of two amino acids on the high DP inulin production capacity of the Vd 1-FFT. Introducing the M19F and H308T mutations in the active site of the Vd 1-FFT greatly reduces its capacity to pro...Continue Reading

References

Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Oct 1, 1998·The Plant Journal : for Cell and Molecular Biology·I M van der MeerA J van Tunen
May 29, 2003·Biophysical Journal·Ingrid J VereykenBen de Kruijff
Jan 22, 2005·The Plant Journal : for Cell and Molecular Biology·Maureen VerhaestAnja Rabijns
Oct 5, 2006·The Plant Journal : for Cell and Molecular Biology·Tita RitsemaSjef Smeekens
Dec 2, 2006·Acta Crystallographica. Section D, Biological Crystallography·Maureen VerhaestAnja Rabijns
Jan 24, 2007·Journal of Applied Microbiology·T van de WieleW Verstraete
Apr 28, 2007·Biochimica Et Biophysica Acta·Dirk K HinchaArnd G Heyer
Aug 30, 2008·The New Phytologist·Lindsey SchroevenWim Van den Ende
Mar 18, 2009·Cellular and Molecular Life Sciences : CMLS·David P LivingstonArnd G Heyer
Sep 22, 2009·The FEBS Journal·Wim Van den EndeKatrien Le Roy
Jan 26, 2013·Journal of Experimental Botany·Darin PeshevWim Van den Ende
Apr 9, 2013·Journal of Experimental Botany·Mohammad Reza Bolouri Moghaddam, Wim Van den Ende
Jul 25, 2013·Frontiers in Plant Science·Wim Van den Ende
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Leonie VogtPaul De Vos
Oct 2, 2014·The Plant Cell·Manuela PeukertAndrea Matros
Apr 22, 2015·The Plant Journal : for Cell and Molecular Biology·Andrea MatrosWim Van den Ende
Apr 24, 2015·Plant Foods for Human Nutrition·Francesca Di Bartolomeo, Wim Van den Ende
Dec 13, 2014·Biotechnology Reports·Linxi YangChibuike C Udenigwe

❮ Previous
Next ❯

Citations

Sep 20, 2017·Plant, Cell & Environment·Maxime VersluysWim Van den Ende
Oct 19, 2019·Biomolecules·Erin DobrangeWim Van den Ende
Jun 3, 2021·International Journal of Molecular Sciences·Silin WuHongbo Zhao

❮ Previous
Next ❯

Software Mentioned

PyMOL
MODELER

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.