1-Methyl-4-Phenylpyridinium-Induced Death of Differentiated SH-SY5Y Neurons Is Potentiated by Cholesterol

Annals of Neurosciences
Anu RajuKochupurackal Parameswarannayar Mohanakumar

Abstract

Hypercholesterolemia is recently considered a risk factor for Parkinson's disease (PD), the most consistent neurodegenerative movement disorder. The study aimed to investigate the effect of exogenous cholesterol on 1-methyl-4-phenylpyridinium (MPP+) parkinsonian neurotoxin-induced cell death, loss of mitochondrial membrane potential, and dopaminergic deficiency in a cellular model of PD. Cholesterol (50 μM) when added in the culture media alone or in combination with MPP+ was studied in SH-SY5Y neuroblastoma cells. There were 4 groups that were studied; SH-SY5Y cells treated with vehicle (control), cells that were treated with 1 mM MPP+ (MPP+), or cholesterol (chol) or both (M + chol). The loss of cell survival was measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Dopamine depletion, microtubule-associated protein 2 (MAP-2), and tyrosine hydroxylase (TH)-positive neuronal loss were determined by HPLC-electrochemical detection and TH immunocytochemistry respectively. Mitochondrial membrane potential in cells stained by tetramethylrhodamine methyl ester dye was analysed by flow cytometry. Cholesterol treatment potentiated a reduction of neuronal viability with loss of TH-positive neurons in cultures...Continue Reading

References

Nov 17, 1994·Annals of the New York Academy of Sciences·C C ChiuehD L Murphy
Dec 1, 1996·Neurology·P Jenner, C W Olanow
Mar 3, 2005·Journal of Lipid Research·Maura HeverinIngemar Björkhem
Feb 6, 2008·Journal of Neurochemistry·Pazit Bar-OnEliezer Masliah
Feb 10, 2009·Current Alzheimer Research·Massimo Stefani, Gianfranco Liguri
Mar 20, 2009·Neurobiology of Aging·Anna PensalfiniCristina Cecchi
Nov 24, 2011·Neuroscience·J Bové, C Perier
Jun 1, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ursula FünfschillingGesine Saher
Sep 28, 2014·Experimental Gerontology·Gurdeep Marwarha, Othman Ghribi
Jul 8, 2015·Frontiers in Aging Neuroscience·Paola GambaGabriella Leonarduzzi
Mar 15, 2016·Current Alzheimer Research·Raquel MarinMario Díaz
Nov 5, 2016·Journal of Lipid Research·Jonas Abdel-KhalikYuqin Wang
Nov 30, 2016·BBA Clinical·Poonam VermaKochupurackal P Mohanakumar
Jan 8, 2017·Molecular Neurobiology·Debashis DuttaKochupurackal P Mohanakumar

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Citations

Jan 10, 2019·Cells·Helena XicoyGerard J M Martens
Nov 8, 2019·Experimental Neurobiology·Uram JinSang Myun Park
Apr 7, 2021·The Journal of Pharmacy and Pharmacology·Tanvi Dayanand Pingale, Girdhari Lal Gupta

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