Oct 16, 1989

10-N nonyl-acridine orange: a fluorescent probe which stains mitochondria independently of their energetic state

Biochemical and Biophysical Research Communications
A MaftahR Julien

Abstract

The specificity of binding of 10-N Nonyl Acridine Orange to mitochondria, and more precisely to inner membranes, is demonstrated by subcellular fractionation of hepatocytes. Unlike Rhodamine 123, which is a preferential marker of the transmembrane potential, Nonyl Acridine Orange binding is essentially independent of the mitochondria energization state although a low uptake of this dye, in response to the potential, may be measured. So 10-N Nonyl acridine orange is an appropriate marker of the mitochondial membrane surface per unit of cell mass.

Mentioned in this Paper

Flow Cytometry
Cytochrome c Oxidase Subunit VIa
Tissue Membrane
Rhodamine 123
N, N, N', N'-Tetramethyl-3,6-Acridinediamine Hydrochloride
Uptake
C.I. Basic Orange 14
Mitochondria, Liver
Fluorescence Spectroscopy
Subcellular Fractionation

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