PMID: 16512431Mar 4, 2006Paper

11B solid-state NMR investigation of the rhamnogalacturonan II-borate complex in plant cell walls

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Tsunenori KamedaJun Ashida

Abstract

The boron in plant cell walls, which is water-insoluble and in the solid state, is solubilized by pectinase digestion to give a dimeric rhamnogalacturonan II-borate (dRG-II-B) complex. To clarify the nondestructive structure of boron present in plant cell walls (as represented by sugar beet fiber), we performed 192- and 96-MHz 11B solid state NMR measurements. The use of a high field magnet frequency of 192-MHz enabled us to observe 11B isotropic chemical shifts at -9.7 and -9.6 ppm for dRG-II-B and sugar beet fiber in the solid state, respectively, demonstrating that the boron in isolated dRG-II-B and in plant cell walls is present as a borate-diol ester (1:2). The observation of the magnetic field dependence of the chemical shift and lineshape for the borate-diol ester (1:2) by quadrupolar interaction suggested that the borate complex had a distorted tetrahedral boron structure.

References

Sep 2, 1996·Carbohydrate Research·P PellerinM A O'Neill
Feb 12, 2005·Macromolecular Bioscience·Tsunenori KamedaMitsuru Yoshida

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Citations

Aug 10, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toshiro Matsunaga, Tadashi Ishii
Oct 4, 2007·Angewandte Chemie·Carole Arnal-HéraultMihail Barboiu

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