1.2 MV/cm pulsed electric fields promote transthyretin aggregate degradation.

Scientific Reports
Gen UrabeSunao Katsuki

Abstract

Numerous theoretical studies have been conducted on the effects of high-voltage electric fields on proteins, but few have produced experimental evidence. To acquire experimental data for the amyloid disassemble theory, we exposed transthyretin aggregates to 1,000 ns 1.26 MV/cm pulsed electric fields (PEFs) to promote transthyretin degradation. The process produced no changes in pH, and the resulting temperature increases were < 1 °C. We conclude that the physical effects of PEFs, rather than thermal or chemical effects, facilitate aggregate degradation.

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Citations

Apr 6, 2021·Computational and Structural Biotechnology Journal·Jiří PrůšaMichal Cifra

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Methods Mentioned

BETA
electrophoresis
Assay
protein assay

Software Mentioned

CST Studio Suite ( SIMULIA )
LAQUAtwin
Image J
ImageJ

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