PMID: 15235068Jul 6, 2004Paper

(123)I-Labeled chitinase as specific radioligand for in vivo detection of fungal infections in mice

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
R SiaensG Slegers

Abstract

Given the scarcity of diagnostic tools for invasive fungal infections, the aim of this project was to develop new, specific radiopharmaceuticals for diagnosis of fungal infections. Chitin, which is expressed in the fungal cell wall but is absent in mammalian and bacterial cells, represents a potentially selective target for development of tracers for fungal infections. ChiB_E144Q (ChiB = chitinase B) from Serratia marcescens was labeled with (123)I, and in vitro and in vivo studies were assessed. (123)I labeling of ChiB_E144Q from S. marcescens by direct iodination was characterized by high-pressure liquid chromatography (HPLC), and stability was evaluated. The in vitro binding properties of the compound to living bacteria, Candida albicans, and Aspergillus fumigatus were examined. Scintigraphy was performed and in vivo characteristics were studied in mice with infected thigh muscles. An average radiochemical yield of 35% was obtained. Radiochemical purity was >97% with a stability of >24 h as determined by HPLC and instant thin-layer chromatography. The average specific activity of the noncarrier-free (123)I-chitinase was 9.25 MBq/ micro g of enzyme. Binding assays showed virtually no binding to Eschericha coli and Staphylococ...Continue Reading

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