PMID: 9524193May 9, 1998Paper

1,25-Dihydroxy vitamin D3 and tri-iodothyronine stimulate the expression of a protein immunologically related to osteocalcin

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
E LuegmayrK Klaushofer

Abstract

Osteocalcin (OC), a bone-specific protein, is a marker of late osteoblastic differentiation. Its expression is influenced by various growth factors and hormones. We investigated the effect of 1, 25-dihydroxy vitamin D3 (D3) and tri-iodothyronine (T3) on OC expression in osteoblast-like MC3T3-E1 cells. A heterologous OC green fluorescence protein (GFP) fusion vector was established and expressed to study possible effects on protein transport. Immunostaining of endogenous OC revealed a significant increase in the percentage of positive cells after D3 and T3 treatment. This was consistent for MC3T3-E1 cells as well as nonosteogenic NIH-3T3 and mammary carcinoma cells, but not for neuroblastoma cells. The perinuclear immunostaining corresponded to the NBD C6 ceramide Golgi staining. Conversely, we found a strong induction of OC in MC3T3-E1 cells at the mRNA and protein levels only with T3 and not with D3. OC mRNA and protein expression was not detected in NIH fibroblasts. OC GFP transfection experiments indicate rapid transport and secretion of OC, because OC GFP was not found to be accumulated at intracellular compartments after hormone treatment. We conclude that the strong perinuclear immunostaining does not represent OC but a p...Continue Reading

References

Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P V HauschkaP M Gallop
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P A PriceN Raman
Apr 1, 1991·Molecular and Cellular Endocrinology·A PirskanenP H Mäenpää
Oct 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G S SteinT A Owen
Aug 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M WeinrebG A Rodan
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J LianG Stein
Mar 1, 1986·Endocrinology·N KuriharaM Kumegawa
Jan 1, 1986·Annual Review of Nutrition·M R Haussler
Aug 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J N BradbeerJ T Triffitt
Jul 1, 1995·The Journal of Nutrition·K KlaushoferM Peterlik
Oct 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J C Fleet, J M Hock
Jun 24, 1996·FEBS Letters·H H Gerdes, C Kaether
Aug 1, 1996·Nature·P DucyG Karsenty
Apr 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S BarilleM Amiot
Jan 3, 1997·The Journal of Biological Chemistry·R ZhangG Karsenty

❮ Previous
Next ❯

Citations

May 23, 2002·DNA and Cell Biology·Monika RumplerKlaus Klaushofer
Jan 23, 2016·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A BrauerW Metzger
Dec 6, 2012·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·W MetzgerM Oberringer
Feb 5, 2002·Molecular Genetics and Metabolism·Clare B HarveyGraham R Williams
Feb 11, 2016·Endocrine Reviews·J H Duncan Bassett, Graham R Williams
May 29, 2018·Wiener medizinische Wochenschrift·Stéphane BlouinKlaus Klaushofer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.