1,25-Dihydroxycholecalciferol enhances butyrate-induced p21(Waf1/Cip1) expression

Biochemical and Biophysical Research Communications
T GaschottJ Stein

Abstract

Butyrate, a short-chain fatty acid produced in the colon, as well as its prodrug tributyrin, reduce proliferation and increase differentiation of colon cancer cells. p21(Waf1/Cip1) and p27(Kip1) are negative regulators of cell cycle and are thought to have a key function in the differentiation of various cell lines. We studied the effects of butyrate on differentiation, VDR expression, as well as on p21(Waf1/Cip1) and p27(Kip1) expression in human colon cancer cells (Caco-2). Butyrate induced cell differentiation, which was further enhanced after addition of 1,25-dihydroxycholecalciferol. Synergistic effect of butyrate and dihydroxycholecalciferol in Caco-2 cells was due to butyrate-induced overexpression of VDR. While butyrate as well as dihydroxycholecalciferol increased p21(Waf1/Cip1) and p27(Kip1) expression, in contrast combined exposure of butyrate and dihydroxycholecalciferol resulted in a synergistic amplification of p21(Waf1/Cip1), but not of p27(Kip1) expression. These data imply that butyrate selectively increases p21(Waf1/Cip1) expression via upregulation of VDR in Caco-2 cells.

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Citations

Oct 29, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Veronika FedirkoJill Joelle Woodard
Oct 26, 2001·Biochemical and Biophysical Research Communications·T GaschottJ Stein
Jan 10, 2002·Biochemical and Biophysical Research Communications·Tanja GaschottJürgen Stein
Feb 1, 2003·Biochemical and Biophysical Research Communications·Dimitrios ZgourasJürgen Stein
Oct 27, 2004·Biochemical and Biophysical Research Communications·Carolin DanielJürgen Stein
Sep 20, 2006·Expert Opinion on Therapeutic Targets·Moray J Campbell, Luciano Adorini
May 2, 2007·Journal of Cellular Biochemistry·Carolin DanielJurgen M Stein
Jun 27, 2012·Annals of the New York Academy of Sciences·Svenja PlögerJoerg R Aschenbach
Jul 5, 2002·The Journal of Nutrition·Freya Wolter, Jürgen Stein
Jan 27, 2006·World Journal of Gastroenterology : WJG·Oliver SchröderJürgen Stein
Jun 17, 2008·Otolaryngologia polska. The Polish otolaryngology·Beata Rostkowska-NadolskaMarcin Fraczek
Oct 31, 2017·Endocrinology and Metabolism Clinics of North America·Moray J Campbell, Donald L Trump

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