PMID: 11331223May 2, 2001Paper

1,25-Dihydroxyvitamin D3 stimulates the production of insulin-like growth factor-binding proteins-2, -3 and -4 in human bone marrow stromal cells

European Journal of Endocrinology
M KveiborgM Kassem

Abstract

1,25-Dihydroxyvitamin D3 (calcitriol) inhibits proliferation and stimulates differentiation of multiple cell types, including osteoblasts. Human (h) bone marrow stromal cells (MSCs) are a homogenous non-hematopoietic population of cells present in the bone marrow and exhibit a less differentiated osteoblastic phenotype. The IGF system, including IGFs-I, and -II and IGF binding proteins (IGFBPs), plays an important role in osteoblast cell proliferation and differentiation. To examine the pattern of expression of the IGF system in hMSCss and its regulation by calcitriol. hMSCs express mRNA of both IGFs-I, and -II and IGFBPs-1 to -6 as shown by RT-PCR and northern blot analysis. As assessed by western ligand blotting (WLB) and western immmunoblot analysis, hMSCs secrete 38-42 kDa IGFBP-3, 24-28 kDa IGFBP-4 and a 33 kDa IGFBP-2. Calcitriol (dose range 10-10 mol/l) exerted no consistent dose-dependent effects on either IGF-I or IGF-II mRNA levels. In contrast, calcitriol treatment increased steady-state mRNA levels of IGFBPs-2, -3 and -4, but had no effect on IGFBP-5 or -6. Similarly, calcitriol increased the secretion of IGFBPs-2, -3 and -4 as determined by WLB. We found no detectable basal IGFBP-3 or IGFBP-4 protease activities in...Continue Reading

Citations

May 5, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Sabine MazerbourgPhilippe Monget
Mar 22, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Maria Lucia Hirata KatayamaMaria Mitzi Brentani
Jan 26, 2006·Stem Cells and Development·Hannjörg KochPhil G Campbell
Dec 8, 2009·Endocrinology·Shuanhu ZhouJulie Glowacki
Mar 8, 2014·BoneKEy Reports·Marjolein van Driel, Johannes P T M van Leeuwen
Feb 13, 2016·Journal of the American Society of Nephrology : JASN·Anna Gooch, Christof Westenfelder
Oct 9, 2007·Archives of Physiology and Biochemistry·A HoeflichD Kiepe
Sep 27, 2015·Molecular and Cellular Endocrinology·Richard C Lindsey, Subburaman Mohan
May 2, 2014·Frontiers in Physiology·Jeroen van de Peppel, Johannes P T M van Leeuwen
Mar 28, 2018·Journal of Molecular Endocrinology·Richard C LindseySubburaman Mohan
Sep 25, 2004·Journal of Cellular Biochemistry·Petra De HaesSiegfried Segaert
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Josef V SilhaLiam J Murphy
Aug 27, 2005·European Journal of Morphology·Laoise M McNamara, Patrick J Prendergast
Apr 3, 2013·Molecular Medicine Reports·Naiwu HuChengbin Zhao

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