PMID: 591725Jan 1, 1977

125I protein A: applications to the quantitative determination of fluid phase and cell-bound IgG

Journal of Immunological Methods
J J LangoneT Borsos


An improved method for the preparation of 125I-labelled Protein A (125I PA) of high specific and functional activity is described. 125I PA has been used in combination with purified rabbit IgG bound to a solid support to develop a competitive binding assay capable of detecting Protein A or human, rabbit and guinea pig IgG at the nanogram level. An optimal set of assay conditions was established and levels of IgG measured in normal human, rabbit and strain-2 guinea pig serum. 125I PA has also been used to detect IgG anti-Forssman antibody bound to sheep erythrocytes and to line-1 and line-10 tumor cells and as an indirect assay for tumor associated antigen in the ascitic fluid of tumor-bearing guinea pigs.


Dec 1, 1984·Clinical Rheumatology·J CalderonJ Gomez-Reyno
Jan 1, 1978·Journal of Immunological Methods·J J Langone
Jan 1, 1979·Journal of Immunological Methods·S J WallerB S Andrews
Jan 1, 1979·Journal of Immunological Methods·R C DuhamelE Meezan
Jan 1, 1980·Journal of Immunological Methods·M D Boyle, J J Langone
Feb 25, 1983·Journal of Immunological Methods·G L Mayers, J Klostergaard
Aug 12, 1983·Journal of Immunological Methods·R LindmarkJ Sjöquist
Apr 15, 1983·Journal of Immunological Methods·K J ReisM D Boyle
Oct 14, 1983·Journal of Immunological Methods·J J LangoneD S Terman
Oct 14, 1983·Journal of Immunological Methods·R TronconeF Ciccimarra
Jul 6, 1984·Journal of Immunological Methods·T Dyrberg, N Billestrup
Aug 3, 1984·Journal of Immunological Methods·H P Wang, P C Mayer
Oct 24, 1985·Journal of Immunological Methods·M T DertzbaughW B Lebherz
Feb 28, 1985·Journal of Immunological Methods·R G Hamilton, N F Adkinson
Mar 16, 1988·Journal of Immunological Methods·K J ReisM D Boyle
Apr 6, 1988·Journal of Immunological Methods·J G Van de WinkelP J Capel
Aug 28, 1991·Journal of Immunological Methods·S M Knicker, A T Profy
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·B M GandhiB N Tandon
Jan 1, 1985·Comparative Immunology, Microbiology and Infectious Diseases·M J LawmanM D Boyle
Sep 1, 1981·Molecular Immunology·T BorsosJ J Langone
Sep 1, 1985·Molecular Immunology·M D BoyleM J Lawman
May 1, 1988·Molecular Immunology·M Kirschfink, T Borsos
May 1, 1983·Immunology Letters·M SandorJ Gergely
Jan 1, 1981·International Journal of Immunopharmacology·D D Wood, M J Staruch
Oct 1, 1991·Biologicals : Journal of the International Association of Biological Standardization·C LucasA B Chen
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G S EisenbarthM Nirenberg
Dec 1, 1992·Scandinavian Journal of Immunology·E GyimesiG Szegedi
Jan 1, 1992·Vox Sanguinis·M KirschfinkD Roelcke
Mar 1, 1994·Occupational and Environmental Medicine·D M HalpinA J Newman Taylor
Jun 1, 1979·Analytical Biochemistry·J J Langone, L Levine
Sep 15, 1981·Analytical Biochemistry·A P Gee, J J Langone
Jan 1, 1989·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·P E VaudauxF A Waldvogel
Feb 1, 1984·European Journal of Clinical Investigation·C HoyouxF Geubelle
Feb 1, 1983·Annals of Neurology·J N WhitakerJ R Mendell
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Mar 1, 1981·The Histochemical Journal·D Glick
Nov 1, 1993·Clinical and Experimental Immunology·M KirschfinkM Loos

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Tumor Antigens
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Immunoassay Method
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