PMID: 591725Jan 1, 1977

125I protein A: applications to the quantitative determination of fluid phase and cell-bound IgG

Journal of Immunological Methods
J J LangoneT Borsos

Abstract

An improved method for the preparation of 125I-labelled Protein A (125I PA) of high specific and functional activity is described. 125I PA has been used in combination with purified rabbit IgG bound to a solid support to develop a competitive binding assay capable of detecting Protein A or human, rabbit and guinea pig IgG at the nanogram level. An optimal set of assay conditions was established and levels of IgG measured in normal human, rabbit and strain-2 guinea pig serum. 125I PA has also been used to detect IgG anti-Forssman antibody bound to sheep erythrocytes and to line-1 and line-10 tumor cells and as an indirect assay for tumor associated antigen in the ascitic fluid of tumor-bearing guinea pigs.

Citations

Dec 1, 1984·Clinical Rheumatology·J CalderonJ Gomez-Reyno
Jan 1, 1978·Journal of Immunological Methods·J J Langone
Jan 1, 1979·Journal of Immunological Methods·S J WallerB S Andrews
Jan 1, 1979·Journal of Immunological Methods·R C DuhamelE Meezan
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Related Concepts

Metazoa
Tumor Antigens
Erythrocytes
Forssman Antigen
Cavia porcellus
IgG2B
Immunoassay Method
Iodine Radioisotopes
Neoplasms, Experimental
Plasma Protein Binding Capacity

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