PMID: 8469773Mar 19, 1993Paper

125I]CGP 42112 binding reveals differences between rat brain and adrenal AT2 receptor binding sites

Regulatory Peptides
R C Speth

Abstract

The AT2 angiotensin II receptor selective ligand CGP42112 was radioiodinated and used to study AT2 receptor binding sites in the rat brain (combined olfactory bulb, septum, thalamus and midbrain) and whole adrenal. The [125I]CGP 42112 binding was of high affinity, saturable and specific in both tissues. Competition studies with nonselective and angiotensin II receptor subtype selective ligands, and evaluation of the effects of the sulfhydryl reducing agent beta-mercaptoethanol, confirmed that [125I]CGP 42112 bound selectively to the AT2 angiotensin II receptor subtype. [125I]CGP 42112 bound with higher affinity in the brain than in the adrenal. beta-Mercaptoethanol enhanced [125I]CGP 42112 binding in the brain, but did not alter its binding in the adrenal. A similar difference in binding affinity for [125I]sarcosine, isoleucine angiotensin II and enhancement of binding affinity by beta-mercaptoethanol was observed in the rat brain and adrenal. These observations suggest that the AT2 angiotensin II receptor subtypes in the brain and adrenal may differ.

References

Feb 28, 1992·Biochemical and Biophysical Research Communications·S P BottariM de Gasparo
Mar 31, 1992·Biochemical and Biophysical Research Communications·S S KakarJ D Neill
Apr 30, 1992·Biochemical and Biophysical Research Communications·T S EltonS Oparil
Feb 22, 1992·The Journal of Comparative Neurology·K SongF A Mendelsohn
May 1, 1992·Neuroendocrinology·B P RoweR C Speth
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Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C SumnersM K Raizada
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Aug 30, 1989·Biochemical and Biophysical Research Communications·S WhitebreadM de Gasparo

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Citations

Nov 10, 1995·Regulatory Peptides·J W Wright, J W Harding
Nov 25, 1997·Brain Research. Brain Research Reviews·J W Wright, J W Harding
Feb 4, 1999·Regulatory Peptides·R C SpethK L Grove
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G M CiuffoJ M Saavedra
Aug 29, 2021·Pharmaceuticals·Liina LaukkanenPlinio C Casarotto

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